Protein Info for NOLOHH_18620 in Escherichia coli ECOR27

Name: ydfJ
Annotation: Putative transporter YdfJ

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 427 transmembrane" amino acids 25 to 49 (25 residues), see Phobius details amino acids 61 to 81 (21 residues), see Phobius details amino acids 87 to 108 (22 residues), see Phobius details amino acids 127 to 152 (26 residues), see Phobius details amino acids 160 to 180 (21 residues), see Phobius details amino acids 223 to 242 (20 residues), see Phobius details amino acids 254 to 272 (19 residues), see Phobius details amino acids 280 to 300 (21 residues), see Phobius details amino acids 307 to 328 (22 residues), see Phobius details amino acids 349 to 368 (20 residues), see Phobius details amino acids 374 to 394 (21 residues), see Phobius details TIGR00883: MFS transporter, metabolite:H+ symporter (MHS) family protein" amino acids 1 to 390 (390 residues), 453.4 bits, see alignment E=3.9e-140 PF07690: MFS_1" amino acids 27 to 314 (288 residues), 104.2 bits, see alignment E=1.1e-33 amino acids 227 to 404 (178 residues), 38.6 bits, see alignment E=9.7e-14 PF00083: Sugar_tr" amino acids 30 to 191 (162 residues), 76.5 bits, see alignment E=3.1e-25 PF13347: MFS_2" amino acids 123 to 334 (212 residues), 27.4 bits, see alignment E=1.9e-10

Best Hits

Swiss-Prot: 100% identical to YDFJ_ECOLI: Putative transporter YdfJ (ydfJ) from Escherichia coli (strain K12)

KEGG orthology group: K08173, MFS transporter, MHS family, metabolite:H+ symporter (inferred from 99% identity to sdy:SDY_1582)

Predicted SEED Role

"Putative transport protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (427 amino acids)

>NOLOHH_18620 Putative transporter YdfJ (Escherichia coli ECOR27)
MDFQLYSLGAALVFHEIFFPESSTAMALILAMGTYGAGYVARIVGAFIFGKMGDRIGRKK
VLFITITMMGICTTLIGVLPTYAQIGVFAPILLVTLRIIQGLGAGAEISGAGTMLAEYAP
KGKRGIISSFVAMGTNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWL
RMNLKESPVFEKVNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYL
VQTLLFNKAIPTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSII
VDKSYAPSTIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGF
GPILAGIFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRA
AQPSRSL