Protein Info for NOLOHH_18030 in Escherichia coli ECOR27

Name: gstA
Annotation: glutathione transferase GstA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02798: GST_N
  • amino acids 1 to 74 (74 residues), 37.5 bits, E=7.1e-13
• PF13409: GST_N_2
  • amino acids 10 to 75 (66 residues), 42.9 bits, E=1.8e-14
• PF13417: GST_N_3
  • amino acids 16 to 79 (64 residues), 28.1 bits, E=6.2e-10
• PF00043: GST_C
  • amino acids 101 to 189 (89 residues), 70.5 bits, E=3.6e-23
• PF14497: GST_C_3
  • amino acids 107 to 193 (87 residues), 37.6 bits, E=6.4e-13
• PF13410: GST_C_2
  • amino acids 124 to 184 (61 residues), 36.6 bits, E=1.2e-12

Best Hits

Swiss-Prot: 100% identical to GSTA_ECOLI: Glutathione S-transferase GstA (gstA) from Escherichia coli (strain K12)

KEGG orthology group: K00799, glutathione S-transferase [EC: 2.5.1.18] (inferred from 100% identity to eco:b1635)

MetaCyc: 100% identical to glutathione S-transferase GstA (Escherichia coli K-12 substr. MG1655)
Glutathione transferase. [EC: 2.5.1.18]

Predicted SEED Role

"Glutathione S-transferase (EC 2.5.1.18)" in subsystem Glutathione: Non-redox reactions (EC 2.5.1.18)

MetaCyc Pathways

• 4-hydroxy-2-nonenal detoxification (1/4 steps found)
• glutathione-mediated detoxification I (3/8 steps found)
• pentachlorophenol degradation (3/10 steps found)
• gliotoxin biosynthesis (1/9 steps found)
• glutathione-mediated detoxification II (1/9 steps found)
• indole glucosinolate activation (intact plant cell) (3/12 steps found)
• camalexin biosynthesis (2/12 steps found)

KEGG Metabolic Maps

• Drug metabolism - cytochrome P450
• Glutathione metabolism
• Metabolism of xenobiotics by cytochrome P450

Isozymes

Compare fitness of predicted isozymes for: 2.5.1.18

Use Curated BLAST to search for 2.5.1.18

Protein Sequence (201 amino acids)

>NOLOHH_18030 glutathione transferase GstA (Escherichia coli ECOR27)
MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYFVVNPKGQVPALLLDDG
TLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIATELHKGFTPLFRPDTPEE
YKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFTVLRWAYAVKLNLEGLEHIAA
FMQRMAERPEVQDALSAEGLK