Protein Info for NOLOHH_14970 in Escherichia coli ECOR27

Name: pdeN
Annotation: cyclic di-GMP phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 518 transmembrane" amino acids 12 to 36 (25 residues), see Phobius details amino acids 185 to 200 (16 residues), see Phobius details amino acids 236 to 258 (23 residues), see Phobius details PF12792: CSS-motif" amino acids 42 to 240 (199 residues), 150.1 bits, see alignment E=6.2e-48 PF00563: EAL" amino acids 267 to 500 (234 residues), 235.2 bits, see alignment E=7.4e-74

Best Hits

Swiss-Prot: 100% identical to PDEN_ECOLI: Probable cyclic di-GMP phosphodiesterase PdeN (pdeN) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 100% identity to eco:b2176)

Predicted SEED Role

"Rtn protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (518 amino acids)

>NOLOHH_14970 cyclic di-GMP phosphodiesterase (Escherichia coli ECOR27)
MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYDTLITDVQKYLDTYFAD
LKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDKKTFCSSATGEMDIPLNEL
IPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSGVFAALNLNLTPSLFYSSRQE
DYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIAGIPLTVRLYADDWTWNDVWYAF
LLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQFYVAYQPVVDTQALRVTGLEVLLR
WRHPVAGEIPPDAFINFAESQKMIVPLTQHLFELIARDAAELEKVLPVGVKFGINIAPDH
LHSESFKADIQKLLTSLPAHHFQIVLEITERDMLKEQEATQLFAWLHSVGVEIAIDDFGT
GHSALIYLERFTLDYLKIDRGFINAIGTETITSPVLDAVLTLAKRLNMLTVAEGVETPEQ
ARWLSERGVNFMQGYWISRPLPLDDFVRWLKKPYTPQW