Protein Info for NOLOHH_14050 in Escherichia coli ECOR27

Name: ypdA
Annotation: two-component system sensor histidine kinase YpdA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 565 transmembrane" amino acids 6 to 26 (21 residues), see Phobius details amino acids 45 to 64 (20 residues), see Phobius details amino acids 75 to 99 (25 residues), see Phobius details amino acids 106 to 128 (23 residues), see Phobius details amino acids 140 to 161 (22 residues), see Phobius details amino acids 173 to 192 (20 residues), see Phobius details PF07694: 5TM-5TMR_LYT" amino acids 28 to 192 (165 residues), 143.5 bits, see alignment E=9.5e-46 PF01590: GAF" amino acids 226 to 343 (118 residues), 28.4 bits, see alignment E=4.4e-10 PF06580: His_kinase" amino acids 359 to 438 (80 residues), 87.9 bits, see alignment E=8.9e-29 PF02518: HATPase_c" amino acids 456 to 553 (98 residues), 42 bits, see alignment E=2.3e-14

Best Hits

Swiss-Prot: 100% identical to YPDA_ECO57: Sensor histidine kinase YpdA (ypdA) from Escherichia coli O157:H7

KEGG orthology group: K02478, two-component system, LytT family, sensor kinase [EC: 2.7.13.3] (inferred from 100% identity to eco:b2380)

MetaCyc: 100% identical to sensor histidine kinase PyrS (Escherichia coli K-12 substr. MG1655)
Histidine kinase. [EC: 2.7.13.3]

Predicted SEED Role

"Autolysis histidine kinase LytS" in subsystem Murein hydrolase regulation and cell death

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3

Use Curated BLAST to search for 2.7.13.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (565 amino acids)

>NOLOHH_14050 two-component system sensor histidine kinase YpdA (Escherichia coli ECOR27)
VHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKELLAVTAIFSLFALFST
WSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRYLIDIGGVTAIPCFITSIL
AGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWAPTTALGIDIVSKIGIPMILG
SVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLPLFRHVNSESLRKVCEIIRDDIH
ADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQAMNYGKIIIKNNDEAHRTPEIHSM
LVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAVGLSQIISTQLEVSRAEQLREMANKAE
LRALQSKINPHFLFNALNAISSSIRLNPDTARQLIFNLSRYLRYNIELKDDEQIDIKKEL
YQIKDYIAIEQARFGDKLTVIYDIDEEVNCCIPSLLIQPLVENAIVHGIQPCKGKGVVTI
SVAECGNRVRIAVRDTGHGIDPKVIERVEANEMPGNKIGLLNVHHRVKLLYGEGLHIRRL
EPGTEIAFYIPNQRTPVASQATLLL