Protein Info for NOLOHH_14035 in Escherichia coli ECOR27

Name: ypdE
Annotation: aminopeptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 831 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details PF00381: PTS-HPr" amino acids 6 to 84 (79 residues), 51.7 bits, see alignment E=1.8e-17 TIGR01417: phosphoenolpyruvate-protein phosphotransferase" amino acids 118 to 674 (557 residues), 507.1 bits, see alignment E=5.9e-156 PF05524: PEP-utilisers_N" amino acids 119 to 234 (116 residues), 49.5 bits, see alignment E=1.2e-16 PF00391: PEP-utilizers" amino acids 261 to 334 (74 residues), 52.2 bits, see alignment E=1e-17 PF02896: PEP-utilizers_C" amino acids 360 to 650 (291 residues), 369.7 bits, see alignment E=2.4e-114 PF00359: PTS_EIIA_2" amino acids 687 to 826 (140 residues), 114 bits, see alignment E=1.4e-36 TIGR00848: PTS system, fructose subfamily, IIA component" amino acids 687 to 812 (126 residues), 139.8 bits, see alignment E=4.6e-45

Best Hits

Swiss-Prot: 99% identical to PTFX_SHIFL: Multiphosphoryl transfer protein (fryA) from Shigella flexneri

KEGG orthology group: K08483, phosphotransferase system, enzyme I, PtsI [EC: 2.7.3.9] K11189, phosphocarrier protein K11201, PTS system, fructose-specific IIA-like component [EC: 2.7.1.69] (inferred from 100% identity to eco:b2383)

Predicted SEED Role

"Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9)" in subsystem Fructose and Mannose Inducible PTS or Fructose utilization or Mannitol Utilization (EC 2.7.3.9)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.69, 2.7.3.9

Use Curated BLAST to search for 2.7.1.69 or 2.7.3.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (831 amino acids)

>NOLOHH_14035 aminopeptidase (Escherichia coli ECOR27)
MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEVTFINHRQNAKADAKSSLALIGTGTLFN
DSCSLNISGSDEEQARRVLEEYIQVRFIDSDSVQPTQAELTAHPLPRSLSRLNPDLLYGN
VLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLATLAEQLNQQLRERDGESKTI
LSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQVCAKLSASASDYLRERVSDIRD
ISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQFLSLDLKNLAGMILEKTGRTSHTL
ILARASAIPVLSGLPLDAIARYAGQPAVLDAQCGVLAINPNDAVSGYYQVAQTLADKRQK
QQAQAAAQLAYSRDNKRIDIAANIGTALEAPGAFANGAEGVGLFRTEMLYMDRDSAPDEQ
EQFEAYQQVLLAAGDKPIIFRTMDIGGDKSIPYLNIPQEENPFLGYRAVRIYPEFAALFR
TQLRAILRAASFGNAQLMIPMVHSLDQILWVKGEIQKAIVELKRDGLRHAETITLGIMVE
VPSVCYIIDHFCDEVDFFSIGSNDMTQYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTT
AHQRGKWVGICGELGGESRYLPLLLGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQ
ACECRSAQEIEALLTAFTPEEDVRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEH
PFELEEDVWQREEIVTTGVGFGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVI
MLTLGANEGMNHVKVFSQLARKLVNKNFRQSLFAAQDAQSILTLLETELTF