Protein Info for NOLOHH_11060 in Escherichia coli ECOR27

Name: mutY
Annotation: A/G-specific adenine glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 TIGR01084: A/G-specific adenine glycosylase" amino acids 5 to 277 (273 residues), 441.3 bits, see alignment E=7.6e-137 PF00730: HhH-GPD" amino acids 36 to 168 (133 residues), 96.5 bits, see alignment E=2.4e-31 PF00633: HHH" amino acids 100 to 127 (28 residues), 31.3 bits, see alignment (E = 2.5e-11) PF10576: EndIII_4Fe-2S" amino acids 192 to 208 (17 residues), 20.7 bits, see alignment (E = 8.1e-08) PF14815: NUDIX_4" amino acids 234 to 343 (110 residues), 86.8 bits, see alignment E=1.8e-28

Best Hits

Swiss-Prot: 100% identical to MUTY_ECOLI: Adenine DNA glycosylase (mutY) from Escherichia coli (strain K12)

KEGG orthology group: K03575, A/G-specific adenine glycosylase [EC: 3.2.2.-] (inferred from 100% identity to eco:b2961)

MetaCyc: 100% identical to adenine DNA glycosylase (Escherichia coli K-12 substr. MG1655)
RXN0-2661 [EC: 3.2.2.31]

Predicted SEED Role

"A/G-specific adenine glycosylase (EC 3.2.2.-)" in subsystem DNA repair, bacterial (EC 3.2.2.-)

Isozymes

Compare fitness of predicted isozymes for: 3.2.2.-

Use Curated BLAST to search for 3.2.2.- or 3.2.2.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (350 amino acids)

>NOLOHH_11060 A/G-specific adenine glycosylase (Escherichia coli ECOR27)
MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQVATVIPYFERFMARFP
TVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGGKFPETFEEVAALPGVGRS
TAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEVENKLWSLSEQVTPAVGVERF
NQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSLYPGKKPKQTLPERTGYFLLLQH
EDEVLLAQRPPSGLWGGLYCFPQFADEESLRQWLAQRQIAADNLTQLTAFRHTFSHFHLD
IVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLAAPVERLLQQLRTGAPV