Protein Info for NOLOHH_07215 in Escherichia coli ECOR27

Name: dgoT
Annotation: D-galactonate transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 430 transmembrane" amino acids 15 to 34 (20 residues), see Phobius details amino acids 53 to 75 (23 residues), see Phobius details amino acids 82 to 108 (27 residues), see Phobius details amino acids 142 to 163 (22 residues), see Phobius details amino acids 169 to 188 (20 residues), see Phobius details amino acids 242 to 263 (22 residues), see Phobius details amino acids 283 to 304 (22 residues), see Phobius details amino acids 316 to 332 (17 residues), see Phobius details amino acids 338 to 361 (24 residues), see Phobius details amino acids 370 to 395 (26 residues), see Phobius details amino acids 402 to 423 (22 residues), see Phobius details PF07690: MFS_1" amino acids 21 to 389 (369 residues), 192 bits, see alignment E=1.5e-60 amino acids 246 to 421 (176 residues), 45.5 bits, see alignment E=5.1e-16 TIGR00881: phosphoglycerate transporter family protein" amino acids 22 to 405 (384 residues), 445.7 bits, see alignment E=1.4e-137 TIGR00893: D-galactonate transporter" amino acids 23 to 423 (401 residues), 493.4 bits, see alignment E=4.8e-152 PF00083: Sugar_tr" amino acids 44 to 420 (377 residues), 33.2 bits, see alignment E=2.8e-12

Best Hits

Swiss-Prot: 100% identical to DGOT_ECOL6: D-galactonate transporter (dgoT) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K08194, MFS transporter, ACS family, D-galactonate transporter (inferred from 100% identity to eco:b3691)

MetaCyc: 100% identical to D-galactonate:H+ symporter (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-16

Predicted SEED Role

"D-galactonate transporter" in subsystem D-galactonate catabolism

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (430 amino acids)

>NOLOHH_07215 D-galactonate transporter (Escherichia coli ECOR27)
MDIPVNAAKPGRRRYLTLVMIFITVVICYVDRANLAVASAHIQEEFGITKAQMGYVFSAF
AWLYTLCQIPGGWFLDRVGSRVTYFIAIFGWSVATLFQGFASGLMSLIGLRAITGIFEAP
AFPTNNRMVTSWFPEHERASAVGFYTSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIG
IIWSLIWFKVYQPPRLTKGISKAELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHR
KLIGVYLGQFAVASTLWFFLTWFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVA
DLLVRKGFSLGFARKTPIICGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSL
VSSLAPMRLIGLTGGVFNFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYI
LLVGDVKRVG