Protein Info for NOLOHH_04445 in Escherichia coli ECOR27

Name: yjfC
Annotation: Putative acid--amine ligase YjfC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF03738: GSP_synth
  • amino acids 14 to 385 (372 residues), 501.3 bits, E=9.7e-155

Best Hits

Swiss-Prot: 98% identical to YJFC_ECOLI: Putative acid--amine ligase YjfC (yjfC) from Escherichia coli (strain K12)

KEGG orthology group: None (inferred from 98% identity to eco:b4186)

MetaCyc: 98% identical to peptide--spermidine ligase YjfC (Escherichia coli K-12 substr. MG1655)
6.3.1.-

Predicted SEED Role

"Similarity with glutathionylspermidine synthase (EC 6.3.1.8), group 1" in subsystem Glutathionylspermidine and Trypanothione (EC 6.3.1.8)

MetaCyc Pathways

• glutathionylspermidine biosynthesis (1/1 steps found)

KEGG Metabolic Maps

• Glutathione metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.3.1.8

Use Curated BLAST to search for 6.3.1.8

Protein Sequence (387 amino acids)

>NOLOHH_04445 Putative acid--amine ligase YjfC (Escherichia coli ECOR27)
MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQIEEQIEKPTAELHQMC
LEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRMDFAWCGNAPVKLLEYNAD
TPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLISRFSELYSREPFYFCCCQDT
DEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLTDLDDNVIQRAFKLYPLEWMMRD
DNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFPGHPNLLASWFEGEKSQIAAGESYV
RKPIYSREGGNVTIFDGQNNVVDHADGDYADEPMIYQAFQPLPRFGDSYTLIGSWIVDDE
ACGMGIREDNTLITKDTSRFVPHYIAG