Protein Info for NOLOHH_03555 in Escherichia coli ECOR27

Name: deoA
Annotation: thymidine phosphorylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02643: thymidine phosphorylase
  • amino acids 2 to 438 (437 residues), 788.2 bits, E=2.2e-241
• TIGR02644: pyrimidine-nucleoside phosphorylase
  • amino acids 5 to 423 (419 residues), 566.9 bits, E=2.4e-174
• PF02885: Glycos_trans_3N
  • amino acids 5 to 67 (63 residues), 68.2 bits, E=6.3e-23
• PF00591: Glycos_transf_3
  • amino acids 79 to 309 (231 residues), 192.9 bits, E=1.2e-60
• PF07831: PYNP_C
  • amino acids 350 to 423 (74 residues), 86.5 bits, E=1.2e-28

Best Hits

Swiss-Prot: 100% identical to TYPH_ECOHS: Thymidine phosphorylase (deoA) from Escherichia coli O9:H4 (strain HS)

KEGG orthology group: K00758, thymidine phosphorylase [EC: 2.4.2.4] (inferred from 99% identity to eco:b4382)

MetaCyc: 99% identical to thymidine phosphorylase (Escherichia coli K-12 substr. MG1655)
Pyrimidine-nucleoside phosphorylase. [EC: 2.4.2.2, 2.4.2.3]; Thymidine phosphorylase. [EC: 2.4.2.2, 2.4.2.3, 2.4.2.4]

Predicted SEED Role

"Thymidine phosphorylase (EC 2.4.2.4)" in subsystem Deoxyribose and Deoxynucleoside Catabolism (EC 2.4.2.4)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleosides degradation (6/6 steps found)
• pyrimidine deoxyribonucleosides degradation (3/3 steps found)
• pyrimidine ribonucleosides degradation (2/2 steps found)
• superpathway of pyrimidine ribonucleosides degradation (4/5 steps found)
• nucleoside and nucleotide degradation (archaea) (4/10 steps found)

KEGG Metabolic Maps

• Drug metabolism - other enzymes
• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.4.2.3

Use Curated BLAST to search for 2.4.2.2 or 2.4.2.3 or 2.4.2.4

Protein Sequence (440 amino acids)

>NOLOHH_03555 thymidine phosphorylase (Escherichia coli ECOR27)
LFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAMTIFFHDMTMPERVSLT
MAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMVAACGGYIPMISGRGLGHT
GGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSLAPADKRFYATRDITATVDSI
PLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSEALAEAIVGVANGAGVRTTALLT
DMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVTMALCVEMLISGKLAKDDAEARAKL
QAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYLPTAMLTKAVYADTEGFVSEMDTRALG
MAVVAMGGGRRQASDTIDYSVGFTDMARLGDQVDGQRPLAVIHAKDENSWQDAAKAVKAA
IKLADKAPESTPTVYRRISE