Protein Info for NOLOHH_03250 in Escherichia coli ECOR27

Name: caiC
Annotation: crotonobetaine/carnitine-CoA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00501: AMP-binding
  • amino acids 17 to 384 (368 residues), 255 bits, E=1.1e-79
• PF13193: AMP-binding_C
  • amino acids 435 to 510 (76 residues), 60.9 bits, E=1.9e-20

Best Hits

Swiss-Prot: 99% identical to CAIC_ECO57: Crotonobetaine/carnitine--CoA ligase (caiC) from Escherichia coli O157:H7

KEGG orthology group: K02182, crotonobetaine/carnitine-CoA ligase [EC: 6.2.1.-] (inferred from 98% identity to eco:b0037)

MetaCyc: 98% identical to carnitine--CoA ligase (Escherichia coli K-12 substr. MG1655)
LCARNCOALIG-RXN [EC: 6.2.1.48]

Predicted SEED Role

"Crotonobetaine carnitine-CoA ligase (EC 6.3.2.-)" (EC 6.3.2.-)

MetaCyc Pathways

• L-carnitine degradation I (5/5 steps found)

KEGG Metabolic Maps

• Alkaloid biosynthesis II
• Benzoate degradation via CoA ligation
• Biosynthesis of plant hormones
• Biosynthesis of siderophore group nonribosomal peptides
• Caprolactam degradation
• Ethylbenzene degradation
• Geraniol degradation
• Limonene and pinene degradation
• Propanoate metabolism
• Tryptophan metabolism
• Tyrosine metabolism
• alpha-Linolenic acid metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.2.1.-, 6.3.2.-

Use Curated BLAST to search for 6.2.1.- or 6.2.1.48 or 6.3.2.-

Protein Sequence (517 amino acids)

>NOLOHH_03250 crotonobetaine/carnitine-CoA ligase (Escherichia coli ECOR27)
MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELNQEINRTANLFYTLGIR
KGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESAWILQNSQACLLVTSAQFY
PMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQPATLCYAPPLSTDDTAEILF
TSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYLTVMPAFHIDCQCTAAMAAFSAG
ATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTLMVQPPSANDRQHRLREVMFYLNLS
EQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPGDKRRWPSIGRAGFCYEAEIRDDHNRP
LPAGEIGEICIKGVPGKTIFKEYFLNPKATAKVLEADGWLHTGDTGYRDEEGFFYFVDRR
CNMIKRGGENVSCVELENIIATHPKIQDIVVVGIKDSIRDEAIKAFVVLNEGETLSEEEF
FRFCEQNMAKFKVPSYLEIRKDLPRNCSGKIIRKNLK