Protein Info for NOLOHH_03240 in Escherichia coli ECOR27

Name: caiA
Annotation: crotonobetainyl-CoA dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 380 PF02771: Acyl-CoA_dh_N" amino acids 6 to 109 (104 residues), 56.4 bits, see alignment E=7.5e-19 PF02770: Acyl-CoA_dh_M" amino acids 123 to 215 (93 residues), 84.3 bits, see alignment E=1.1e-27 PF00441: Acyl-CoA_dh_1" amino acids 227 to 376 (150 residues), 162.1 bits, see alignment E=2.2e-51 PF08028: Acyl-CoA_dh_2" amino acids 245 to 356 (112 residues), 58.4 bits, see alignment E=1.9e-19

Best Hits

Swiss-Prot: 100% identical to CAIA_ECOUT: Crotonobetainyl-CoA reductase (caiA) from Escherichia coli (strain UTI89 / UPEC)

KEGG orthology group: K08297, crotonobetainyl-CoA dehydrogenase [EC: 1.3.99.-] (inferred from 100% identity to eco:b0039)

MetaCyc: 100% identical to crotonobetainyl-CoA reductase (Escherichia coli K-12 substr. MG1655)
CROBETREDUCT-RXN [EC: 1.3.8.13]

Predicted SEED Role

"Crotonobetainyl-CoA dehydrogenase (EC 1.3.99.-)" (EC 1.3.99.-)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.99.-

Use Curated BLAST to search for 1.3.8.13 or 1.3.99.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (380 amino acids)

>NOLOHH_03240 crotonobetainyl-CoA dehydrogenase (Escherichia coli ECOR27)
MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVKALADMGIDSLLIPEEH
GGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGTQEQIDKIMAFRGTGKQMW
NSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSAYTPYIVVMARDGASPDKPVY
TEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELDEKDMFGREGNGFNRVKEEFDHE
RFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRFQLIQEKFAHMAIKLNSMKNMLYEA
AWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQVLGGVGIAGNHRISRFWRDLRVDRVS
GGSDEMQILTLGRAVLKQYR