Protein Info for NOLOHH_00270 in Escherichia coli ECOR27

Annotation: Endonuclease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 163 signal peptide" amino acids 1 to 21 (21 residues), see Phobius details PF00565: SNase" amino acids 53 to 149 (97 residues), 91.1 bits, see alignment E=3.1e-30

Best Hits

Swiss-Prot: 55% identical to YFI3_ECOLX: Uncharacterized endonuclease from Escherichia coli

KEGG orthology group: None (inferred from 87% identity to sbc:SbBS512_0052)

Predicted SEED Role

"DNA topoisomerase III (EC 5.99.1.2)" in subsystem DNA topoisomerases, Type I, ATP-independent (EC 5.99.1.2)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.2

Use Curated BLAST to search for 5.99.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (163 amino acids)

>NOLOHH_00270 Endonuclease (Escherichia coli ECOR27)
MKLTKYVFFFVTIFSGSLFAAEIQGRVIRVLDGDTIEIKTLPAKIVVYEVPIRVRLINID
APEKKQPFGRWSTNQLKALLAGQSVTVSYTQTDRYGRIIGHVFTTNGTDASRFMVKSGAA
WVYERYNADESLPALQREAQEQKRGLWADSNPVPPWEWRHKQN