Protein Info for NIAGMN_26295 in Escherichia coli ECRC102

Annotation: exonuclease VIII

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 866 PF06630: Exonuc_VIII" amino acids 2 to 178 (177 residues), 326 bits, see alignment E=1.1e-101 PF12684: DUF3799" amino acids 615 to 850 (236 residues), 90.9 bits, see alignment E=1.1e-29

Best Hits

Swiss-Prot: 99% identical to RECE_ECOLI: Exodeoxyribonuclease 8 (recE) from Escherichia coli (strain K12)

KEGG orthology group: K10906, exodeoxyribonuclease VIII [EC: 3.1.11.-] (inferred from 100% identity to ece:Z2409)

MetaCyc: 99% identical to Rac prophage; exonuclease VIII, ds DNA exonuclease, 5' --> 3' specific (Escherichia coli K-12 substr. MG1655)
3.1.11.-

Predicted SEED Role

"Exodeoxyribonuclease VIII (EC 3.1.11.-)" (EC 3.1.11.-)

Isozymes

Compare fitness of predicted isozymes for: 3.1.11.-

Use Curated BLAST to search for 3.1.11.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (866 amino acids)

>NIAGMN_26295 exonuclease VIII (Escherichia coli ECRC102)
MSTKPLFLLRKAKKSSGEPDVVLWASNDFESTCATLDYLIVKSGKKLSSYFKAVATNFPV
VNDLPAEGEIDFTWSERYQLSKDSMTWELKPGAAPDNAHYQGNTNVNGEDMTEIEENMLL
PISGQELPIRWLAQHGSEKPVTHVSRDGLQALHIARAEELPAVTALAVSHKTSLLDPLEI
RELHKLVRDTDKVFPNPGNSNLGLITAFFEAYLNADYTDRGLLTKEWMKGNRVSHITRTA
SGANAGGGNLTDRGEGFVHDLTSLARDVATGVLARSMDLDIYNLHPAHAKRIEEIIAENK
PPFSVFRDKFITMPGGLDYSRAIVVASVKEAPIGIEVIPAHVTEYLNKVLTETDHANPDP
EIVDIACGRSSAPMPQRVTEEGKQDDEEKPQPSGTTAVEQGEAETMEPDATEHHQDTQPL
DAQSQVNSVDAKYQELRAELHEARKNIPSKNPVDADKLLAASRGEFVDGISDPNDPKWVK
GIQTRDCVYQNQPETEKTSPDMNQPEPVVQQEPEIACNACGQTGGDNCPDCGAVMGDATY
QETFDEESQVEAKENDPEEMEGAEHPHNENAGSDPHRDCSDETGEVADPVIVEDIEPGIY
YGISNENYHAGPGVSKSQLDDIADTPALYLWRKNAPVDTTKTKTLDLGTAFHCRVLEPEE
FSNRFIVAPEFNRRTNSGKEEEKAFLRECASTGKTVITAEEGRKIELMYQSVMALPLGQW
LVESAGHAESSIYWEDPETAILCRCRPDKIIPEFHWIMDVKTTADIQRFKTAYYDYRYHV
QDAFYSDGYEAQFGVQPTFVFLVASTTIECGRYPVEIFMMGEEAKLAGQLEYHRNLRTLA
DCLNTDEWPAIKTLSLPRWAKEYAND