Protein Info for NIAGMN_25435 in Escherichia coli ECRC102

Name: dosP
Annotation: oxygen-sensing cyclic-di-GMP phosphodiesterase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 413 TIGR00229: PAS domain S-box protein" amino acids 18 to 126 (109 residues), 77 bits, see alignment E=7e-26 amino acids 141 to 256 (116 residues), 54.2 bits, see alignment E=8.1e-19 PF13426: PAS_9" amino acids 24 to 124 (101 residues), 53.4 bits, see alignment E=9.3e-18 amino acids 148 to 250 (103 residues), 45.7 bits, see alignment E=2.5e-15 PF00989: PAS" amino acids 24 to 124 (101 residues), 38.1 bits, see alignment E=4.8e-13 amino acids 142 to 248 (107 residues), 36.7 bits, see alignment E=1.3e-12 PF13188: PAS_8" amino acids 141 to 189 (49 residues), 27.8 bits, see alignment 6.2e-10 PF08448: PAS_4" amino acids 142 to 253 (112 residues), 38.9 bits, see alignment E=3.1e-13

Best Hits

KEGG orthology group: K13243, c-di-GMP-specific phosphodiesterase [EC: 3.1.4.52] (inferred from 99% identity to ecc:c1918)

Predicted SEED Role

"Heme-regulated cyclic AMP phosphodiesterase (EC 3.1.4.-)" in subsystem Putative hemin transporter or cAMP signaling in bacteria (EC 3.1.4.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.4.-, 3.1.4.52

Use Curated BLAST to search for 3.1.4.- or 3.1.4.52

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (413 amino acids)

>NIAGMN_25435 oxygen-sensing cyclic-di-GMP phosphodiesterase (Escherichia coli ECRC102)
MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKLWGYKREEVIGNNIDML
IPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIWTRFALSKVSAEGKVYYLA
LVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQCNRAFTEMFGYCISEASGMQ
PDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRTGEKIWIKASISPVYDVLAHLQN
LVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGEIICRNIESVLNESHVSLFALRNGM
PIHWASSSHGAEVQNAQSWSATIRQRDGAPAGILQIKTSSGAETSAFIERVADISQHMAA
LALEQEKSRQHIEQLIQFDPMTGLPNRNNLHNYLDDLVDKAVSPVSVLTIFRM