Protein Info for NIAGMN_23730 in Escherichia coli ECRC102

Name: putA
Annotation: trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF21775: PutA_1st
  • amino acids 11 to 43 (33 residues), 75.1 bits, see alignment (E = 5.8e-25)
• PF18327: PRODH
  • amino acids 89 to 136 (48 residues), 64.6 bits, see alignment (E = 2e-21)
• PF14850: Pro_dh-DNA_bdg
  • amino acids 148 to 259 (112 residues), 155.2 bits, E=2.1e-49
• PF01619: Pro_dh
  • amino acids 268 to 569 (302 residues), 360.7 bits, E=1.5e-111
• TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase
  • amino acids 610 to 1112 (503 residues), 871.6 bits, E=7.3e-267
• PF00171: Aldedh
  • amino acids 659 to 1103 (445 residues), 365.1 bits, E=1.2e-112

Best Hits

Swiss-Prot: 100% identical to PUTA_ECOLI: Bifunctional protein PutA (putA) from Escherichia coli (strain K12)

MetaCyc: 100% identical to fused DNA-binding transcriptional repressor / proline dehydrogenase / 1-pyrroline-5-carboxylate dehydrogenase PutA (Escherichia coli K-12 substr. MG1655)
RXN0-7008 [EC: 1.5.5.2]; RXN-14116 [EC: 1.5.5.2, 1.2.1.88]

Predicted SEED Role

"Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

• L-proline degradation I (2/3 steps found)
• proline to cytochrome bo oxidase electron transfer (1/2 steps found)
• L-citrulline biosynthesis (5/8 steps found)
• L-arginine degradation I (arginase pathway) (1/3 steps found)
• superpathway of L-citrulline metabolism (7/12 steps found)
• ethene biosynthesis II (microbes) (1/4 steps found)
• L-N^δ-acetylornithine biosynthesis (3/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.5.2 or 1.5.99.8

Protein Sequence (1320 amino acids)

>NIAGMN_23730 trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase (Escherichia coli ECRC102)
MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQLENSDTLPELPALLSGA
ANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRPETEAVSMLLEQARLPQPV
AEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQEGVALMCLAEALLRIPDKAT
RDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGKLVSTHNEASLSRSLNRIIGKSG
EPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLEEKGFRYSYDMLGEAALTAADAQAY
MVSYQQAIHAIGKASNGRGIYEGPGISIKLSALHPRYSRAQYDRVMEELYPRLKSLTLLA
RQYDIGINIDAEEADRLEISLDLLEKLCFEPELAGWNGIGFVIQAYQKRCPLVIDYLIDL
ATRSRRRLMIRLVKGAYWDSEIKRAQMDGLEGYPVYTRKVYTDVSYLACAKKLLAVPNLI
YPQFATHNAHTLAAIYQLAGQNYYPGQYEFQCLHGMGEPLYEQVTGKVADGKLNRPCRIY
APVGTHETLLAYLVRRLLENGANTSFVNRIADTSLPLDELVADPVTAVEKLAQQEGQTGL
PHPKIPLPRDLYGHGRDNSAGLDLANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEM
SPVINPAEPKDIVGYVREATPREVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQ
MQQLIGILVREAGKTFSNAIAEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNF
PLAIFTGQIAAALAAGNSVLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGRGETVGA
QLTGDDRVRGVMFTGSTEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQ
VVIDVLASAFDSAGQRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVI
DSEAKANIERHIQTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVF
GPVLHVVRYNRNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVG
AVVGVQPFGGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALT
QPLNALREWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDE
QDALTQLAAVLAVGSQVLWPDDALHRQLVKALPSTVSERIQLAKAENITAQPFDAVIFHG
DSDQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG