Protein Info for NIAGMN_23080 in Escherichia coli ECRC102

Name: appA
Annotation: bifunctional acid phosphatase/4-phytase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00328: His_Phos_2
  • amino acids 31 to 376 (346 residues), 191.4 bits, E=1.6e-60

Best Hits

Swiss-Prot: 97% identical to PPA_ECOLI: Periplasmic AppA protein (appA) from Escherichia coli (strain K12)

KEGG orthology group: K01093, 4-phytase / acid phosphatase [EC: 3.1.3.2 3.1.3.26] (inferred from 100% identity to etw:ECSP_1149)

MetaCyc: 97% identical to periplasmic phosphoanhydride phosphatase/multiple inositol-polyphosphate phosphatase (Escherichia coli K-12 substr. MG1655)
3.6.1.15,3.6.5.6,3.6.5.5,3.6.5.4,3.6.5.3,3.6.5.2,3.6.5.1,3.6.1.-,3.6.1.5 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6]; Multiple inositol-polyphosphate phosphatase. [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6, 3.1.3.62]; 3.1.3.62 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6, 3.1.3.62]; 3.1.3.62 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6, 3.1.3.62]; 3.1.3.62 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6, 3.1.3.62]; 3.1.3.62 [EC: 3.6.1.15, 3.6.1.5, 3.6.5.1, 3.6.5.2, 3.6.5.3, 3.6.5.4, 3.6.5.5, 3.6.5.6, 3.1.3.62]

Predicted SEED Role

"Phosphoanhydride phosphohydrolase (EC 3.1.3.2) (pH 2.5 acid phosphatase) (AP) / 4- phytase (EC 3.1.3.26)" (EC 3.1.3.2, EC 3.1.3.26)

MetaCyc Pathways

• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (18/18 steps found)
• superpathway of pyrimidine deoxyribonucleotides de novo biosynthesis (E. coli) (14/14 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis I (9/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis III (8/9 steps found)
• pyrimidine deoxyribonucleotides de novo biosynthesis IV (6/7 steps found)
• phosphate acquisition (1/1 steps found)
• pyrimidine deoxyribonucleotides biosynthesis from CTP (6/8 steps found)
• UTP and CTP dephosphorylation I (5/7 steps found)
• UTP and CTP dephosphorylation II (1/3 steps found)
• superpathway of D-myo-inositol (1,4,5)-trisphosphate metabolism (1/6 steps found)
• phytate degradation II (1/8 steps found)
• phytate degradation I (4/14 steps found)

KEGG Metabolic Maps

• Inositol phosphate metabolism
• Purine metabolism
• Pyrimidine metabolism
• Riboflavin metabolism
• Thiamine metabolism
• gamma-Hexachlorocyclohexane degradation

Isozymes

Compare fitness of predicted isozymes for: 3.1.3.2, 3.6.1.15

Use Curated BLAST to search for 3.1.3.2 or 3.1.3.26 or 3.1.3.62 or 3.6.1.15 or 3.6.1.5 or 3.6.5.1 or 3.6.5.2 or 3.6.5.3 or 3.6.5.4 or 3.6.5.5 or 3.6.5.6

Protein Sequence (434 amino acids)

>NIAGMN_23080 bifunctional acid phosphatase/4-phytase (Escherichia coli ECRC102)
MKAILIPFLSLLIPLTPQSAFAQSEPEPELKLESVVIVSRHGVRAPTKATQLMQDVTPDA
WPNWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLTKKGCPQPGQVAIIADVDERTRKT
GEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNANVTDAILSRAGGSIADFT
GHRQTAFRELERVLNFPQSNLCLNREKQDESCSLTQALPSELKVSADNVSLTGAVSLASM
LTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQFYLLQRTPEVARSRATPLLDLIM
IALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGGALELNWTLPGQPDNTPPGGELVFE
RWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNTPPGEVKLTLAGCEERNAQGMCSLAGF
TQIVNEARIPACSL