Protein Info for NIAGMN_20990 in Escherichia coli ECRC102

Name: mqo
Annotation: malate dehydrogenase (quinone)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06039: Mqo
  • amino acids 31 to 518 (488 residues), 800.9 bits, E=5.7e-245
• TIGR01320: malate dehydrogenase (acceptor)
  • amino acids 32 to 516 (485 residues), 716.5 bits, E=7.7e-220
• PF01266: DAO
  • amino acids 33 to 304 (272 residues), 37.2 bits, E=3.8e-13

Best Hits

Swiss-Prot: 100% identical to MQO_ECO45: Probable malate:quinone oxidoreductase (mqo) from Escherichia coli O45:K1 (strain S88 / ExPEC)

KEGG orthology group: K00116, malate dehydrogenase (quinone) [EC: 1.1.5.4] (inferred from 100% identity to eco:b2210)

MetaCyc: 100% identical to malate:quinone oxidoreductase (Escherichia coli K-12 substr. MG1655)
MALATE-DEHYDROGENASE-ACCEPTOR-RXN [EC: 1.1.5.4]

Predicted SEED Role

"Malate:quinone oxidoreductase (EC 1.1.5.4)" in subsystem TCA Cycle (EC 1.1.5.4)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (25/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)

KEGG Metabolic Maps

• Pyruvate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.1.5.4

Protein Sequence (548 amino acids)

>NIAGMN_20990 malate dehydrogenase (quinone) (Escherichia coli ECRC102)
MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSATLGTYLRELEPEWSMT
MVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISIEKAVAINEAFQISRQFWA
HQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAALQQSSLFRGMRYSEDHAQIKE
WAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIASLQKKSNFSLQLSSEVRALKRND
DNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSE
NPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDL
MSSTTTSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQ
RVQIIKRDADKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSS
PQWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQ
KEVADIAL