Protein Info for NIAGMN_20670 in Escherichia coli ECRC102

Name: nuoN
Annotation: NADH-quinone oxidoreductase subunit NuoN

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01770: proton-translocating NADH-quinone oxidoreductase, chain N
  • amino acids 9 to 475 (467 residues), 498.1 bits, E=1.5e-153
• PF00361: Proton_antipo_M
  • amino acids 122 to 420 (299 residues), 308.1 bits, E=3.1e-96

Best Hits

Swiss-Prot: 100% identical to NUON_ECOSE: NADH-quinone oxidoreductase subunit N (nuoN) from Escherichia coli (strain SE11)

KEGG orthology group: K00343, NADH dehydrogenase I subunit N [EC: 1.6.5.3] (inferred from 100% identity to eco:b2276)

MetaCyc: 100% identical to NADH:quinone oxidoreductase subunit N (Escherichia coli K-12 substr. MG1655)
NADH-DEHYDROG-A-RXN [EC: 7.1.1.2]; 7.1.1.- [EC: 7.1.1.2]

Predicted SEED Role

"NADH-ubiquinone oxidoreductase chain N (EC 1.6.5.3)" in subsystem Respiratory Complex I (EC 1.6.5.3)

MetaCyc Pathways

• NADH to cytochrome bd oxidase electron transfer I (2/2 steps found)
• NADH to dimethyl sulfoxide electron transfer (2/2 steps found)
• NADH to fumarate electron transfer (2/2 steps found)
• NADH to hydrogen peroxide electron transfer (2/2 steps found)
• NADH to trimethylamine N-oxide electron transfer (2/2 steps found)
• nitrate reduction VIII (dissimilatory) (2/2 steps found)
• aerobic respiration III (alternative oxidase pathway) (2/3 steps found)
• NAD(P)/NADPH interconversion (4/6 steps found)
• NADH to cytochrome bo oxidase electron transfer I (1/2 steps found)
• aerobic respiration I (cytochrome c) (2/4 steps found)
• Fe(II) oxidation (1/6 steps found)

KEGG Metabolic Maps

• Oxidative phosphorylation

Isozymes

Compare fitness of predicted isozymes for: 1.6.5.3

Use Curated BLAST to search for 1.6.5.3 or 7.1.1.2

Protein Sequence (485 amino acids)

>NIAGMN_20670 NADH-quinone oxidoreductase subunit NuoN (Escherichia coli ECRC102)
MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIGLNAALVSLWFVGQAGA
MDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNKDEFYLLVLIAALGGILLA
NANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYTILSAAASSFLLFGMALVYAQ
SGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKLSLVPFHLWTPDVYQGAPAPVST
FLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAIIAFASIIFGNLMALSQTNIKRLLGY
SSISHLGYLLVALIALQTGEMSMEAVGVYLAGYLFSSLGAFGVVSLMSSPYRGPDADSLF
SYRGLFWHRPILAAVMTVMMLSLAGIPMTLGFIGKFYVLAVGVQAHLWWLVGAVVVGSAI
GLYYYLRVAVSLYLHAPEQPGRDAPSNWQYSAGGIVVLISALLVLVLGVWPQPLISIVRL
AMPLM