Protein Info for NIAGMN_19665 in Escherichia coli ECRC102

Name: hyfB
Annotation: hydrogenase 4 subunit B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 672 transmembrane" amino acids 6 to 27 (22 residues), see Phobius details amino acids 34 to 54 (21 residues), see Phobius details amino acids 80 to 100 (21 residues), see Phobius details amino acids 111 to 129 (19 residues), see Phobius details amino acids 135 to 154 (20 residues), see Phobius details amino acids 166 to 185 (20 residues), see Phobius details amino acids 205 to 223 (19 residues), see Phobius details amino acids 243 to 262 (20 residues), see Phobius details amino acids 273 to 296 (24 residues), see Phobius details amino acids 303 to 326 (24 residues), see Phobius details amino acids 337 to 363 (27 residues), see Phobius details amino acids 383 to 404 (22 residues), see Phobius details amino acids 425 to 452 (28 residues), see Phobius details amino acids 473 to 495 (23 residues), see Phobius details amino acids 532 to 551 (20 residues), see Phobius details amino acids 652 to 671 (20 residues), see Phobius details PF00361: Proton_antipo_M" amino acids 136 to 425 (290 residues), 181.9 bits, see alignment E=8.4e-58

Best Hits

Swiss-Prot: 99% identical to HYFB_ECOLI: Hydrogenase-4 component B (hyfB) from Escherichia coli (strain K12)

KEGG orthology group: K12137, hydrogenase-4 component B [EC: 1.-.-.-] (inferred from 99% identity to eco:b2482)

Predicted SEED Role

No annotation

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-

Use Curated BLAST to search for 1.-.-.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (672 amino acids)

>NIAGMN_19665 hydrogenase 4 subunit B (Escherichia coli ECRC102)
MDALQLLTWSLILYLFASLASLFLLGLDRLAIKLSGITSLVGGVIGIISGITQLHEGVTL
VARFATPFDFADLTLRMDSLSAFMVLVISLLVVVCSLYSLTYMREYEGKGAAAMGFFMNI
FIASMVALLVMDNAFWFIVLFEMMSLSSWFLVIARQDKTSINAGMLYFFIAHAGSVLIMI
AFLLMGRESGSLDFASFRTLSLSPGLASAVFLLAFFGFGAKAGMMPLHSWLPRAHPAAPS
HASALMSGVMVKIGIFGILKVAMDLLAQTGLPLWWGILVMAIGAISALLGVLYALAEQDI
KRLLAWSTVENVGIILLAVGVAMVGLSLHDPLLTVVGLLGALFHLLNHALFKGLLFLGAG
AIISRLHTHDMEKMGALAKRMPWTAAACLIGCLAISALPPLNGFISEWYTWQSLFSLSRV
EAVALQLAGPIAMVMLAVTGGLAVMCFVKMYGITFCGAPRSTHAEEAQEVPNTMIVAMLL
LAALCVLIALSASWLAPKIMHIAHAFTNTPPVTVASGIALVPGTFHTQVTPSLLLLLLLA
MPLLPGLYWLWCRSRRAAFRRTGDAWACGYGWENAMAPSGNGVMQPLRVVFSALFRLRQQ
LDPTLRLNKGLAHVTARAQSTEPFWDERVIRPIVSATQRLAKEIQHLQSGDFRLYCLYVV
AALVVLLIAIAV