Protein Info for NIAGMN_17210 in Escherichia coli ECRC102

Name: scpA
Annotation: methylmalonyl-CoA mutase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01642: MM_CoA_mutase
  • amino acids 28 to 539 (512 residues), 778.1 bits, E=3.8e-238
• TIGR00641: methylmalonyl-CoA mutase N-terminal domain
  • amino acids 28 to 547 (520 residues), 935.5 bits, E=6.7e-286
• TIGR00640: methylmalonyl-CoA mutase C-terminal domain
  • amino acids 583 to 713 (131 residues), 215.8 bits, E=1.7e-68
• PF02310: B12-binding
  • amino acids 586 to 701 (116 residues), 69.8 bits, E=1.9e-23

Best Hits

Swiss-Prot: 99% identical to SCPA_ECOLI: Methylmalonyl-CoA mutase (scpA) from Escherichia coli (strain K12)

KEGG orthology group: K01847, methylmalonyl-CoA mutase [EC: 5.4.99.2] (inferred from 100% identity to ece:Z4254)

MetaCyc: 99% identical to methylmalonyl-CoA mutase (Escherichia coli K-12 substr. MG1655)
Methylmalonyl-CoA mutase. [EC: 5.4.99.2]

Predicted SEED Role

"Methylmalonyl-CoA mutase (EC 5.4.99.2)" in subsystem Propionyl-CoA to Succinyl-CoA Module or Serine-glyoxylate cycle (EC 5.4.99.2)

MetaCyc Pathways

• conversion of succinate to propanoate (3/3 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (10/13 steps found)
• pyruvate fermentation to propanoate I (5/7 steps found)
• propanoyl CoA degradation I (2/3 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (7/10 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (12/17 steps found)
• 2-oxobutanoate degradation I (2/4 steps found)
• L-glutamate degradation VIII (to propanoate) (7/11 steps found)
• 3-hydroxypropanoate cycle (7/13 steps found)
• methylaspartate cycle (11/19 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• superpathway of the 3-hydroxypropanoate cycle (7/18 steps found)
• superpathway of L-methionine salvage and degradation (5/16 steps found)
• crotonyl-CoA/ethylmalonyl-CoA/hydroxybutyryl-CoA cycle (engineered) (2/14 steps found)

KEGG Metabolic Maps

• Propanoate metabolism
• Valine, leucine and isoleucine degradation

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.2

Protein Sequence (714 amino acids)

>NIAGMN_17210 methylmalonyl-CoA mutase (Escherichia coli ECRC102)
MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADLDNLEVTGTLPGLPPYV
RGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGLSVAFDLATHRGYDSDNPR
VAGDVGKTGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAVLPVLAFYIIAAEEQGVTPDK
LTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCSGNMPRFNTISISGYHMGEAGAN
CVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFFFGIGMDLFMNVAMLRAARYLWSEA
VSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNVIRTTIEALAATLGGTQSLHTNAFDEA
LGLPTDFSARIARNTQIIIQEESELCRTVDPLAGSYYIESLTDQIVKQARAIIQQIDEAG
GMAKAIEAGLPKRMIEEASAREQSLIDQGKRVIVGVNKYKLDHEDETDVLEIDNVMVRNE
QIASLERIRATRDDAAVTAALNALTHAAQHNENLLAAAVNAARVRATLGEISDALEAAFD
RYLVPSQCVTGVIAQSYHQSEKSASEFDAIVAQTEQFLADNGRRPRILIAKMGLDGHDRG
AKVIASAYSDLGFDVDLSPMFSTPEEIARLAVENDVHVVGASSLAAGHKTLIPELVEALK
KWGREDICVVAGGVIPPQDYAFLQERGVAAIYGPGTPMLDSVRDVLNLISQHHD