Protein Info for NIAGMN_10530 in Escherichia coli ECRC102

Name: phnN
Annotation: ribose 1,5-bisphosphokinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR02322: phosphonate metabolism protein/1,5-bisphosphokinase (PRPP-forming) PhnN
  • amino acids 3 to 178 (176 residues), 246.8 bits, E=5.4e-78
• PF13238: AAA_18
  • amino acids 6 to 139 (134 residues), 40.1 bits, E=2.7e-14

Best Hits

Swiss-Prot: 100% identical to PHNN_ECO57: Ribose 1,5-bisphosphate phosphokinase PhnN (phnN) from Escherichia coli O157:H7

KEGG orthology group: K05774, ribose 1,5-bisphosphokinase [EC: 2.7.4.23] (inferred from 100% identity to eco:b4094)

MetaCyc: 100% identical to ribose 1,5-bisphosphate phosphokinase (Escherichia coli K-12 substr. MG1655)
Ribose 1,5-bisphosphate phosphokinase. [EC: 2.7.4.23]

Predicted SEED Role

"ATP-binding protein PhnN; Guanylate kinase (EC 2.7.4.8)" (EC 2.7.4.8)

MetaCyc Pathways

• superpathway of histidine, purine, and pyrimidine biosynthesis (46/46 steps found)
• superpathway of purine nucleotides de novo biosynthesis II (26/26 steps found)
• superpathway of purine nucleotides de novo biosynthesis I (21/21 steps found)
• superpathway of purine nucleotide salvage (13/14 steps found)
• (aminomethyl)phosphonate degradation (8/8 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis II (8/8 steps found)
• superpathway of guanosine nucleotides de novo biosynthesis I (6/6 steps found)
• methylphosphonate degradation I (5/5 steps found)
• guanosine ribonucleotides de novo biosynthesis (4/4 steps found)
• dZTP biosynthesis (3/5 steps found)
• glyphosate degradation III (4/7 steps found)

KEGG Metabolic Maps

• Pentose phosphate pathway
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.4.8

Use Curated BLAST to search for 2.7.4.23 or 2.7.4.8

Protein Sequence (185 amino acids)

>NIAGMN_10530 ribose 1,5-bisphosphokinase (Escherichia coli ECRC102)
MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASAGSENHIALSEQEFFTR
AGQNLLALSWHANGLYYGVGVEIDLWLHAGFDVLVNGSRAHLPQARARYQSALLPICLQV
SPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNNDGSLRQSVDTLLTLIHQKEK
HHACL