Protein Info for NIAGMN_09375 in Escherichia coli ECRC102

Name: iadA
Annotation: beta-aspartyl-peptidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 390 TIGR01975: beta-aspartyl peptidase" amino acids 9 to 388 (380 residues), 623.1 bits, see alignment E=1.1e-191 PF01979: Amidohydro_1" amino acids 266 to 374 (109 residues), 25.9 bits, see alignment E=5.7e-10

Best Hits

Swiss-Prot: 100% identical to IADA_ECOLI: Isoaspartyl dipeptidase (iadA) from Escherichia coli (strain K12)

KEGG orthology group: K01305, beta-aspartyl-dipeptidase (metallo-type) [EC: 3.4.19.-] (inferred from 100% identity to eco:b4328)

MetaCyc: 100% identical to isoaspartyl dipeptidase (Escherichia coli K-12 substr. MG1655)
Beta-aspartyl-peptidase. [EC: 3.4.19.5]

Predicted SEED Role

"Isoaspartyl dipeptidase (EC 3.4.19.5) @ Asp-X dipeptidase" (EC 3.4.19.5)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 3.4.19.5

Use Curated BLAST to search for 3.4.19.- or 3.4.19.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (390 amino acids)

>NIAGMN_09375 beta-aspartyl-peptidase (Escherichia coli ECRC102)
MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPSDIVPDCTVVDLSGQIL
CPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVGLLGTDSISRHPESLLAKT
RALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIGVKCAISDHRSAAPDVYHLAN
MAAESRVGGLLGGKPGVTVFHMGDSKKALQPIYDLLENCDVPISKLLPTHVNRNVPLFEQ
ALEFARKGGTIDITSSIDEPVAPAEGIVRAVQAGIPLARVTLSSDGNGSQPFFDDEGNLT
HIGVAGFETLLETVQVLVKDYDFSISDALRPLTSSVAGFLNLTGKGEILPGNDADLLVMT
PELRIEQVYARGKLMVKDGKACVKGTFETA