Protein Info for NIAGMN_08835 in Escherichia coli ECRC102

Name: ribF
Annotation: bifunctional riboflavin kinase/FAD synthetase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF06574: FAD_syn
  • amino acids 13 to 168 (156 residues), 196.6 bits, E=3.5e-62
• TIGR00083: riboflavin biosynthesis protein RibF
  • amino acids 19 to 307 (289 residues), 409.9 bits, E=3.2e-127
• PF01467: CTP_transf_like
  • amino acids 20 to 171 (152 residues), 21.7 bits, E=3e-08
• PF01687: Flavokinase
  • amino acids 185 to 307 (123 residues), 143.7 bits, E=5.1e-46

Best Hits

Swiss-Prot: 100% identical to RIBF_ECOLI: Bifunctional riboflavin kinase/FMN adenylyltransferase (ribF) from Escherichia coli (strain K12)

KEGG orthology group: K11753, riboflavin kinase / FMN adenylyltransferase [EC: 2.7.1.26 2.7.7.2] (inferred from 100% identity to eco:b0025)

MetaCyc: 100% identical to bifunctional riboflavin kinase / FMN adenylyltransferase (Escherichia coli K-12 substr. MG1655)
FAD synthetase. [EC: 2.7.7.2]; Riboflavin kinase. [EC: 2.7.7.2, 2.7.1.26]

Predicted SEED Role

"Riboflavin kinase (EC 2.7.1.26) / FMN adenylyltransferase (EC 2.7.7.2)" in subsystem Riboflavin, FMN and FAD metabolism (EC 2.7.1.26, EC 2.7.7.2)

MetaCyc Pathways

• flavin biosynthesis I (bacteria and plants) (9/9 steps found)
• flavin biosynthesis III (fungi) (8/9 steps found)
• flavin salvage (2/2 steps found)
• 5,6-dimethylbenzimidazole biosynthesis I (aerobic) (2/3 steps found)
• flavin biosynthesis II (archaea) (6/10 steps found)
• roseoflavin biosynthesis (1/7 steps found)
• adenosylcobalamin biosynthesis II (aerobic) (17/33 steps found)

KEGG Metabolic Maps

• Riboflavin metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.1.26 or 2.7.7.2

Protein Sequence (313 amino acids)

>NIAGMN_08835 bifunctional riboflavin kinase/FAD synthetase (Escherichia coli ECRC102)
MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGRKRNLPVMVMLFEPQPL
ELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAALTAQNFISDLLVKHLRVKF
LAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEGGVRISSTAVRQALADDNLAL
AESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQVSPVKGVYAVEVLGLGEKPLPGV
ANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVLRKKIRNEQRFASLDELKAQIARDE
LTAREFFGLTKPA