Protein Info for NIAGMN_06865 in Escherichia coli ECRC102

Name: lacZ
Annotation: beta-galactosidase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 100 200 300 400 500 600 700 800 900 1024 PF02837: Glyco_hydro_2_N" amino acids 52 to 219 (168 residues), 250.5 bits, see alignment E=2.1e-78 PF00703: Glyco_hydro_2" amino acids 221 to 334 (114 residues), 50.3 bits, see alignment E=9.8e-17 PF02836: Glyco_hydro_2_C" amino acids 336 to 630 (295 residues), 421.5 bits, see alignment E=4.6e-130 PF16353: LacZ_4" amino acids 637 to 723 (87 residues), 75.8 bits, see alignment E=6.6e-25 PF02929: Bgal_small_N" amino acids 754 to 1020 (267 residues), 200.6 bits, see alignment E=8.9e-63

Best Hits

Swiss-Prot: 100% identical to BGAL_ECO57: Beta-galactosidase (lacZ) from Escherichia coli O157:H7

KEGG orthology group: K01190, beta-galactosidase [EC: 3.2.1.23] (inferred from 97% identity to enc:ECL_03691)

MetaCyc: 97% identical to beta-galactosidase (Escherichia coli K-12 substr. MG1655)
5.4.1.-; Lactase. [EC: 3.2.1.108]; 3.2.1.- [EC: 3.2.1.108]; 3.2.1.- [EC: 3.2.1.108]

Predicted SEED Role

"Beta-galactosidase (EC 3.2.1.23)" in subsystem Galactosylceramide and Sulfatide metabolism or Lactose and Galactose Uptake and Utilization or Lactose utilization (EC 3.2.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.2.1.23

Use Curated BLAST to search for 3.2.1.108 or 3.2.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (1024 amino acids)

>NIAGMN_06865 beta-galactosidase (Escherichia coli ECRC102)
MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTNRPSQQLRSLNGEWQ
FVWFPAPEAVPESWLECDLPDADTVVVPSNWQMHGYDAPIYTNVTYPITVNPPFVPTENP
TGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLLSEFDLSAFLRA
GENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATLFNDDFSRAV
LEAEVQMYGELRDELRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRVTLGLNVENPK
LWSAEIPNIYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKPLLIRGVNRHEH
HPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGLYVVDEANIETHG
MVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGANHDALYRWIKSVD
PSRPVQYEGGGADTSATDIICPMYARVDEDQPFPAVPKWSIKKWLSLPGEMRPLILCEYA
HAMGNSLGGFAKYWQAFRQYPRLQGGFVWDLVDQSLIKYDENGNPWSAYGGDFGDTPNDR
QFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGRTIEVTSEYLFRHSDNELLHWTVALD
GKPLASGEVPLDVAPQGKQVIELPELPRLESTGQLWLTVHVVQPNATAWSEAGHISAWQQ
WRLAENLSVTLPSAPHAIPQLTTSETDFCIELDNKRWQFNRQSGFLSQMWIGDKKQLLTP
LRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEAALLQCTADTLADAVLITTV
HAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLG
LGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNI
SRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLV
WCQK