Protein Info for NIAGMN_05765 in Escherichia coli ECRC102

Name: cusS
Annotation: Cu(+)/Ag(+) sensor histidine kinase CusS

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 482 transmembrane" amino acids 12 to 36 (25 residues), see Phobius details amino acids 164 to 180 (17 residues), see Phobius details amino acids 187 to 207 (21 residues), see Phobius details PF21085: CusS" amino acids 8 to 180 (173 residues), 149.2 bits, see alignment E=2.8e-47 TIGR01386: heavy metal sensor kinase" amino acids 9 to 479 (471 residues), 521.5 bits, see alignment E=1.1e-160 PF00672: HAMP" amino acids 210 to 257 (48 residues), 41.6 bits, see alignment 3.2e-14 PF00512: HisKA" amino acids 262 to 326 (65 residues), 48.9 bits, see alignment E=1.4e-16 PF02518: HATPase_c" amino acids 371 to 481 (111 residues), 93 bits, see alignment E=4.1e-30 PF14501: HATPase_c_5" amino acids 376 to 471 (96 residues), 22.3 bits, see alignment E=2.6e-08

Best Hits

Swiss-Prot: 100% identical to CUSS_ECO57: Sensor histidine kinase CusS (cusS) from Escherichia coli O157:H7

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 100% identity to ece:Z0708)

MetaCyc: 98% identical to sensor histidine kinase CusS (Escherichia coli K-12 substr. MG1655)
Protein-histidine pros-kinase. [EC: 2.7.13.1]

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.1 or 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (482 amino acids)

>NIAGMN_05765 Cu(+)/Ag(+) sensor histidine kinase CusS (Escherichia coli ECRC102)
MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFAEQDINDLKEISATLER
VLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSPGAPDIREFARDAIPDKDA
RGGEVFLLSGPTMMMPGHGHGHMEHSNWRMISLPVGPLVDGKPIYTLYIALSIDFHLHYI
NDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSRQIQNITSKDLDVRLDPQTVPIE
LEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTPITNLITQTEIALSQSRSQKELEDV
LYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKKMLNLADEVGKVFDFFEALAEDRGVEL
QFVGDECQVAGDPLMLRRALSNLLSNALRYTPPGEAIVVRCQTVDHLVQVIVENPGTPIA
PEHLPRLFDRFYRVDPSRQRKGEGSGIGLAIVKSIVVAHKGTVAVTSNARGTRFVIVLPE
RG