Protein Info for NIAGMN_05515 in Escherichia coli ECRC102

Name: citF
Annotation: citrate lyase subunit alpha

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 510 TIGR01584: citrate lyase, alpha subunit" amino acids 36 to 510 (475 residues), 810 bits, see alignment E=3.6e-248 PF04223: CitF" amino acids 43 to 508 (466 residues), 931.1 bits, see alignment E=7.3e-285

Best Hits

Swiss-Prot: 99% identical to CILA_ECOLI: Citrate lyase alpha chain (citF) from Escherichia coli (strain K12)

KEGG orthology group: K01643, citrate lyase subunit alpha / citrate CoA-transferase [EC: 2.8.3.10 4.1.3.6] (inferred from 99% identity to eco:b0615)

MetaCyc: 99% identical to citrate lyase alpha subunit (Escherichia coli K-12 substr. MG1655)

Predicted SEED Role

"Citrate lyase alpha chain (EC 4.1.3.6)" (EC 4.1.3.6)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 4.1.3.6

Use Curated BLAST to search for 2.8.3.10 or 4.1.3.6

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (510 amino acids)

>NIAGMN_05515 citrate lyase subunit alpha (Escherichia coli ECRC102)
MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDRKLCANLEEAIRRSGLQ
DGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLILASSSLSDCHAPLVEHIRQGVVTRIY
TSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNIDVAFLGVPSCDEFGNANGYT
GKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASIEQDQVDLIVKVDRVGDAAKIGA
GATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQTGTGGASLAVTLFLEDKMRSRDIRA
DFALGGITATMVDLHEKGLIRKLLDVQSFDSHAAQSLARNPNHIEISANQYANWGSKGAS
VDRLDVVVLSALEIDTQFNVNVLTGSDGVLRGASGGHCDTAIASALSIIVAPLVRGRIPT
LVDNVLTCITPGSSVDILVTDHGIAVNPARPELAERLQEAGIKVVSIEWLRERARLLTGE
PQPIEFTDRVVAVVRYRDGSVIDVVHQVKE