Protein Info for MPMX26_03013 in Acinetobacter radioresistens SK82

Annotation: Phosphoenolpyruvate carboxylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 86% identical to CAPP_ACIBT: Phosphoenolpyruvate carboxylase (ppc) from Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377)

KEGG orthology group: K01595, phosphoenolpyruvate carboxylase [EC: 4.1.1.31] (inferred from 86% identity to abm:ABSDF3641)

Predicted SEED Role

"Phosphoenolpyruvate carboxylase (EC 4.1.1.31)" in subsystem Fermentations: Mixed acid or Pyruvate metabolism I: anaplerotic reactions, PEP (EC 4.1.1.31)

MetaCyc Pathways

partial TCA cycle (obligate autotrophs) (7/8 steps found)
CO₂ fixation into oxaloacetate (anaplerotic) (2/2 steps found)
mixed acid fermentation (12/16 steps found)
nitrogen remobilization from senescing leaves (6/8 steps found)
C4 photosynthetic carbon assimilation cycle, NAD-ME type (8/11 steps found)
ethene biosynthesis V (engineered) (18/25 steps found)
C4 photosynthetic carbon assimilation cycle, NADP-ME type (4/7 steps found)
C4 photosynthetic carbon assimilation cycle, PEPCK type (8/14 steps found)
formaldehyde assimilation I (serine pathway) (7/13 steps found)
gluconeogenesis II (Methanobacterium thermoautotrophicum) (9/18 steps found)
Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 4.1.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (894 amino acids)

>MPMX26_03013 Phosphoenolpyruvate carboxylase (Acinetobacter radioresistens SK82)
MIQQIDAPLREDVRLLGNLLGETLKLHAGQDLFNQIEQIRALAKGARDGQKEAEKQLEEL
FSTLKDEEILPLTRAFSHFLNFANIAEQYHLVRSRRQHEFDENAPSPNPLDHLFQKFKQK
DISADALYQQVCELKIELVLTAHPTEVSRRTLIQKYDGINQCLSRLDQQKLTPHERQSVL
DEMKQLICSAWQTDEIRQHRPTPIDEAKWGFTTIEQTLWNAVPKFIHELDQLVLEHCGRP
LPLEVAPIRFASWMGGDRDGNPNVTHQVTQEVLWLSRWQAADLYVRDIEALRWELSIQNC
SEEMSEALGYTHPEPYREYLRQTRERLRATRHWLAQKLQGQDADASLVIHSKDELLQPLL
LCYRSLMACNLPEIANGRLLDFIRRVNCFGIELLKLDVRQESGRHRQAISAITEYLGLGN
FDTWTEQARQNFLLQELQSKRPLLPKHFNEPAQSLILHPDVQEVFATMQTLAEQPSESLG
AYIISMAEYPSDVLAVLLLQKEAGIQRPLRVVPLFETLKDLDGAAATMATLFNMHWYRQH
IQGKHEVMIGYSDSAKDAGFMSANWAQYRAQEELTAVARQHAVQLTLFHGRGGSISRGGA
PTQQALFSQPPGSISGAIRVTEQGEMIRFKFGVEGIALQNLEIYTAATLEATLLPPPEPK
AEWRELMHCMTDLSVQVYRQTVRENPHFVQYLRTVTPELELQMLPLGSRPAKRKVSGGIE
SLRAIPWVFAWTQIRLMLPAWLGTGAALNQVLDQGQKELLDEMLQQWPYFQTLIDMLEMV
LSKADAHVALYYEAHLTDDPELKALGQELRQRLQDAVQTLLALKGESKLLSHNEVLDQSM
RVRKPYLLPLHLLQAKLMKRRRIYLAEQQLEHTPVDHALMVSIAGIAAGLRNTG