Protein Info for MPMX26_02796 in Acinetobacter radioresistens SK82

Annotation: Bifunctional (p)ppGpp synthase/hydrolase SpoT

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 699 PF13328: HD_4" amino acids 32 to 179 (148 residues), 133.8 bits, see alignment E=9.1e-43 PF01966: HD" amino acids 52 to 148 (97 residues), 38.1 bits, see alignment E=3.4e-13 PF02824: TGS" amino acids 381 to 440 (60 residues), 62.2 bits, see alignment 7.7e-21 PF28438: RelA_RIS" amino acids 551 to 609 (59 residues), 48.2 bits, see alignment 1.4e-16

Best Hits

KEGG orthology group: K01139, guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase [EC: 3.1.7.2] (inferred from 83% identity to aci:ACIAD3326)

Predicted SEED Role

"GTP pyrophosphokinase (EC 2.7.6.5), (p)ppGpp synthetase II / Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase (EC 3.1.7.2)" in subsystem Stringent Response, (p)ppGpp metabolism (EC 2.7.6.5, EC 3.1.7.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.7.6.5

Use Curated BLAST to search for 2.7.6.5 or 3.1.7.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (699 amino acids)

>MPMX26_02796 Bifunctional (p)ppGpp synthase/hydrolase SpoT (Acinetobacter radioresistens SK82)
MPGEEVSQARLQLKMIIDAYLSPDDVERVLAACDFADLAHTGITRKSGEPYILHPIAVTC
ILAHMRLDSETLMAALMHDVVEDTDFSKEDIKEKFGPVVAELVDGVTKLTHSSDKQYNKA
ASFRKILQATLQDPRVIIIKLADRYHNMTTLDALRPDKRARIAQETFDIFVPMARIVGMN
EMADNLEHLCYQNLDLDMFDNVQEALKQTKPKRCQFQAIWEQKLGELLKAYHLEGRIKKK
DNNIELLRHFVKNEMNLQELTHSHAFEIILQSIADCDRLVEALEQNFQVLQFEDHIRRPL
PGGNQSLLMKLKGEQTTLSLTIQTELMRKAARFGVVLGESAPQACRSAIQASMQNLNTLI
DGECAKTTFNDLLDYLHQEKIWVYTPHGHLHELPQGATVVDFAYAASLFLGNHAVGAKIN
GEIRPLSTPLVSGQVVEIITDVLATPNPDWLSFINTQKARRALQNILKDQDIDEQRLVGQ
QALNRALKMFNRSIKDLSDADWVDLLQWRHIEHKESLFEQIAVGDLLPQLVANHLYAQEQ
ADEEYNSQRLIQGTDGVDVKYAHCCNPVLGDPIQGHLSRRGLIVHRARCNNLMHEQHLHP
ETIMPLHWNSESEEEVSFTAYLCIDMAMDDEQISDLIYQCRKAKTGVEMVQSQDDRTYVN
IVVNNRRQIAQIIRDLRMHFGFPRISRLSMPVALVQNAS