Protein Info for MPMX26_02783 in Acinetobacter radioresistens SK82

Annotation: tRNA (guanine-N(1)-)-methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 249 signal peptide" amino acids 1 to 16 (16 residues), see Phobius details PF01746: tRNA_m1G_MT" amino acids 1 to 230 (230 residues), 284.2 bits, see alignment E=3.4e-89 TIGR00088: tRNA (guanine(37)-N(1))-methyltransferase" amino acids 1 to 238 (238 residues), 302.4 bits, see alignment E=9e-95

Best Hits

Swiss-Prot: 89% identical to TRMD_ACIBT: tRNA (guanine-N(1)-)-methyltransferase (trmD) from Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377)

KEGG orthology group: K00554, tRNA (guanine-N1-)-methyltransferase [EC: 2.1.1.31] (inferred from 88% identity to abc:ACICU_03361)

MetaCyc: 53% identical to tRNA m1G37 methyltransferase (Escherichia coli K-12 substr. MG1655)
RXN-12458 [EC: 2.1.1.228]

Predicted SEED Role

"tRNA (Guanine37-N1) -methyltransferase (EC 2.1.1.31)" in subsystem Ribosome biogenesis bacterial or Wyeosine-MimG Biosynthesis (EC 2.1.1.31)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.1.1.228 or 2.1.1.31

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (249 amino acids)

>MPMX26_02783 tRNA (guanine-N(1)-)-methyltransferase (Acinetobacter radioresistens SK82)
MFFAVITLFPEMFEAITSFGISGRAAKRDLMQIHCINPREFAEGNYKRVDERPFGGGPGM
VMMAEPLAKAIHHAKELAVQAGAVHVPVVYMSPQGKTLNEAAVQNFVQYDGLILLCGRYE
GVDERLIQKYVDQEWSIGDYVLSGGELPAMVLLDSIIRRLPGAMSDEQSHVQDSFVDGLL
DCPQYTKPDHFEGMHVPEILKSGHHANIEKWRFLQRYQRTLERRPELVEKVELTKQQKKW
LQDSQRQTK