Protein Info for MPMX26_02642 in Acinetobacter radioresistens SK82

Annotation: tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 635 transmembrane" amino acids 279 to 296 (18 residues), see Phobius details PF05430: Methyltransf_30" amino acids 117 to 240 (124 residues), 126.9 bits, see alignment E=1.3e-40 TIGR03197: tRNA U-34 5-methylaminomethyl-2-thiouridine biosynthesis protein MnmC, C-terminal domain" amino acids 278 to 578 (301 residues), 204.2 bits, see alignment E=1.8e-64 PF01266: DAO" amino acids 279 to 604 (326 residues), 114.6 bits, see alignment E=2.3e-36 PF13450: NAD_binding_8" amino acids 281 to 314 (34 residues), 28.2 bits, see alignment (E = 5.7e-10)

Best Hits

Predicted SEED Role

"tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase (EC 2.1.1.61)" (EC 2.1.1.61)

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.61

Use Curated BLAST to search for 2.1.1.61

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (635 amino acids)

>MPMX26_02642 tRNA 5-methylaminomethyl-2-thiouridine biosynthesis bifunctional protein MnmC (Acinetobacter radioresistens SK82)
MSYPIQTAALDWQLIDNIEVPVSRQFGDVYFSKDNGLLETRHVFLNGNDLTERLATLNDF
EYFCVGETGFGTGLNILALWQLWQQVRPDNHSHLHAISVEKFPLGKPDLIRALKAWPELA
PLAEQLLQQYPLLIAGCHRLSFPEERFSLDLWLGDAHDMFPLMEKTASVNAWFLDGFAPS
CNPDMWEEQVLNHIVRLSDHGTTFASFSVAGVLKRGLRSHGIQVTRPRGFGHKREMLKAI
WQPSVADAATEKQEKPESLSSGQLLPRIAVKTTHRQKQIAVIGAGIAGLSVAWSFAQRGH
QVKLFDRNAPLAGASGNPLALLNPKLSQIEKSHIHLMTITWQYALNHYQQFRAFRPLQIH
QMALKESEQLLSLAEQYPHELLTIQTAQCHPLSTQFESLRLNRAGGVSPHLLRDQILEHS
LIQFAQAAVEHIIPEAQDTVLYDANGKSLGKFDHVVVCCARQSQSLLSNCPTLRPIRGQV
SWVNNTVQPLNMQEAISYGGYCLQFDPENLLLGASFLPGREDDEVLAEDHQHNAELLAKV
FPEYAATLPPIEAWQGRASIRAQTMDYFPLLGKMGSDTEIYTFSGLGSKGFLYAPLCSEI
LAALVFKEACPVPASVVQKLNPQRFQKRLKQQRNS