Protein Info for MPMX26_02517 in Acinetobacter radioresistens SK82

Annotation: Proline--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 569 (569 residues), 822.3 bits, E=1.3e-251
• PF00587: tRNA-synt_2b
  • amino acids 96 to 462 (367 residues), 121.3 bits, E=7.8e-39
• PF04073: tRNA_edit
  • amino acids 256 to 379 (124 residues), 70.7 bits, E=1.8e-23
• PF03129: HGTP_anticodon
  • amino acids 478 to 570 (93 residues), 67.3 bits, E=1.7e-22

Best Hits

Swiss-Prot: 91% identical to SYP_ACIAD: Proline--tRNA ligase (proS) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 91% identity to aci:ACIAD0782)

MetaCyc: 68% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• thiocoraline biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• anguibactin biosynthesis (1/8 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• pyochelin biosynthesis (1/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• sulfazecin biosynthesis (1/16 steps found)
• phosalacine biosynthesis (1/25 steps found)
• phosphinothricin tripeptide biosynthesis (1/25 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16

Protein Sequence (571 amino acids)

>MPMX26_02517 Proline--tRNA ligase (Acinetobacter radioresistens SK82)
MRASRFLFATLRETPNDAEVISHQLMLRAGMIRKLASGLYTWLPMGVRVLNKVEAIVREE
MNRAGALETFMPVTQPASLWEESGRYVQYGPELLRFNDRHTNPFVLGPTHEEVITDLARN
ELKSYKQLPINFYQIQTKFRDEVRPRFGVMRSREFIMKDAYSFHVDQASLQETYDVMYET
YCRIFSRLGLDFRPVQADTGSIGGSGSHEFHVLASSGEDDIAFSTESDYAANIEMAEAIL
VGERAAPTQALEIVDTPNQKTIADVSTFLNTDPAHSVKALLVQGIADEKGHSPVIALFLR
GDHELNEIKAEKHPLIAAPLTFATEEQIAALGLTAGFIGPQGLVEKGLTVIVDRAASVLS
DFVAGANEADKHATGVNWERDASFTEVYDLRNVVEGDPSPDGKGTLQIKRGIEVGHIFQL
GKKYSEALGCKVLGEDGKPLTVTMGCYGIGVTRVIASAIEQNYDEKGIIWPAAIAPFEVA
IVPMNAHKSPRTLEAAEALYIELQAQGFDVLLDDRNERPGVKFSDLELTGIPHRIVIGEK
GLDAGTFEYKGRTDIESVNINKDELLAKLAK