Protein Info for MPMX26_02486 in Acinetobacter radioresistens SK82

Annotation: Formamidopyrimidine-DNA glycosylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 272 PF01149: Fapy_DNA_glyco" amino acids 1 to 111 (111 residues), 109.2 bits, see alignment E=2.9e-35 TIGR00577: DNA-formamidopyrimidine glycosylase" amino acids 1 to 266 (266 residues), 297.8 bits, see alignment E=3.7e-93 PF06831: H2TH" amino acids 127 to 217 (91 residues), 106 bits, see alignment E=1.2e-34

Best Hits

Swiss-Prot: 79% identical to FPG_ACIBS: Formamidopyrimidine-DNA glycosylase (mutM) from Acinetobacter baumannii (strain SDF)

KEGG orthology group: K10563, formamidopyrimidine-DNA glycosylase [EC: 3.2.2.23 4.2.99.18] (inferred from 80% identity to acd:AOLE_03065)

MetaCyc: 46% identical to DNA-formamidopyrimidine glycosylase (Escherichia coli K-12 substr. MG1655)
DNA-formamidopyrimidine glycosylase. [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; 3.2.2.23 [EC: 3.2.2.23]; RXN-21279 [EC: 3.2.2.23]

Predicted SEED Role

"Formamidopyrimidine-DNA glycosylase (EC 3.2.2.23)" in subsystem DNA Repair Base Excision (EC 3.2.2.23)

Isozymes

Compare fitness of predicted isozymes for: 4.2.99.18

Use Curated BLAST to search for 3.2.2.23 or 4.2.99.18

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (272 amino acids)

>MPMX26_02486 Formamidopyrimidine-DNA glycosylase (Acinetobacter radioresistens SK82)
MPELPEVETTKKSLEPLLQQKVMSVEVREPRLRWAIPQDIQKLQGQKLIRLNRRSKYILA
QFEHDTMLWHLGMSGSFRLCEPATELRKHDHLIISFENIQLRYHDPRRFGCILWLNHETQ
NKLIDPLGPEPLSEAFNTEYLVNKLKNKQVGIKVALMDNHVVVGVGNIYATESLFNTRIH
PAQPASSLSYEQIDQLVIEIKRILKFAIELGGSTLRDYSNAAGENGYFQQTLLAYGREGE
MCINCETPLQNIRLGQRASVFCPECQPLRTVK