Protein Info for MPMX26_02477 in Acinetobacter radioresistens SK82

Annotation: Dual-specificity RNA pseudouridine synthase RluA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00005: pseudouridine synthase, RluA family
  • amino acids 11 to 220 (210 residues), 194.7 bits, E=1.1e-61
• PF00849: PseudoU_synth_2
  • amino acids 24 to 171 (148 residues), 116.3 bits, E=7.5e-38

Best Hits

Swiss-Prot: 51% identical to RLUA_VIBPA: Ribosomal large subunit pseudouridine synthase A (rluA) from Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633)

KEGG orthology group: K06177, ribosomal large subunit pseudouridine synthase A [EC: 5.4.99.12] (inferred from 76% identity to acd:AOLE_18880)

Predicted SEED Role

"Ribosomal large subunit pseudouridine synthase A (EC 4.2.1.70)" in subsystem Ribosome biogenesis bacterial (EC 4.2.1.70)

MetaCyc Pathways

• pseudouridine degradation (1/2 steps found)

KEGG Metabolic Maps

• Pyrimidine metabolism

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.70, 5.4.99.12

Use Curated BLAST to search for 4.2.1.70 or 5.4.99.12

Protein Sequence (221 amino acids)

>MPMX26_02477 Dual-specificity RNA pseudouridine synthase RluA (Acinetobacter radioresistens SK82)
MALNQPFIYAPPQQPLSIIYEDDDLIIIDKPAGLLSVPGRLPEHQDSAYLRLLEKYPHAK
ITHRLDMATSGILMFAKHRDAEVAISKMFQARTVTKRYVALIQGQLNEQGSIDVPLITDW
ENRPRQIVHFELGKSAKTLFQVLQYHEEHDMTRVLLTPITGRSHQLRVHMQYIGHPIMGD
KLYHPEPGGFYLRRMALHASYLAFQQPLSKDLIEINMAIPF