Protein Info for MPMX26_02135 in Acinetobacter radioresistens SK82

Annotation: Glutamate 5-kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01027: glutamate 5-kinase
  • amino acids 15 to 375 (361 residues), 427 bits, E=3.3e-132
• PF00696: AA_kinase
  • amino acids 15 to 244 (230 residues), 179.7 bits, E=8e-57
• PF01472: PUA
  • amino acids 286 to 359 (74 residues), 71.4 bits, E=4.7e-24

Best Hits

Swiss-Prot: 91% identical to PROB_ACIAD: Glutamate 5-kinase (proB) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K00931, glutamate 5-kinase [EC: 2.7.2.11] (inferred from 91% identity to abm:ABSDF0980)

MetaCyc: 41% identical to glutamate 5-kinase (Escherichia coli K-12 substr. MG1655)
Glutamate 5-kinase. [EC: 2.7.2.11]

Predicted SEED Role

"Glutamate 5-kinase (EC 2.7.2.11) / RNA-binding C-terminal domain PUA" in subsystem Proline Synthesis (EC 2.7.2.11)

MetaCyc Pathways

• L-proline biosynthesis I (from L-glutamate) (4/4 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• L-ornithine biosynthesis II (2/3 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Urea cycle and metabolism of amino groups

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.2.11

Protein Sequence (377 amino acids)

>MPMX26_02135 Glutamate 5-kinase (Acinetobacter radioresistens SK82)
MIEVVDGQRQLKACKRIVVKIGSSLLTANGQGLDLNAISHWANQIADLHKAGHEIILVSS
GAVAEGMVRMKLSSRPTDLPSLQACAAIGQMGLIQTWSSVLEKHGIQTAQVLLTHDDLAD
RRRYLNSCDALQHLIDWQVVPVINENDTVSTDEIRFGDNDTLAAMVAGQVHADLLIILTD
QQGMFDSDPRSNPQAKLLSTVRALDDSLFEMAGGGGVLGRGGMVTKVRAARLAARSGCPT
LIASGESDNVLSRLMSGEMLGTLFATDKDRMTAHQQWLAAHLQTAGRLVIDDGAIEAIRE
KHRSLLPVGVRAVEGHFNRGDVVECVDSRGKRIAVGRVNFSSRSAEIVKGLSSNKVYQVL
GEARSLEMIHRNHMAIY