Protein Info for MPMX26_02120 in Acinetobacter radioresistens SK82

Annotation: Acyl-coenzyme A dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 830 signal peptide" amino acids 1 to 17 (17 residues), see Phobius details transmembrane" amino acids 32 to 61 (30 residues), see Phobius details amino acids 280 to 297 (18 residues), see Phobius details amino acids 379 to 399 (21 residues), see Phobius details amino acids 588 to 605 (18 residues), see Phobius details amino acids 618 to 635 (18 residues), see Phobius details PF02771: Acyl-CoA_dh_N" amino acids 126 to 233 (108 residues), 70 bits, see alignment E=4.6e-23 PF02770: Acyl-CoA_dh_M" amino acids 238 to 331 (94 residues), 33.8 bits, see alignment E=6.3e-12 PF00441: Acyl-CoA_dh_1" amino acids 362 to 503 (142 residues), 50 bits, see alignment E=7.3e-17 PF09317: ACDH_C" amino acids 516 to 800 (285 residues), 313.3 bits, see alignment E=3.9e-97

Best Hits

Swiss-Prot: 48% identical to FADE_SALTY: Acyl-coenzyme A dehydrogenase (fadE) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K06445, acyl-CoA dehydrogenase [EC: 1.3.99.-] (inferred from 79% identity to aci:ACIAD2277)

MetaCyc: 48% identical to acyl-CoA dehydrogenase (Escherichia coli K-12 substr. MG1655)
RXN-17775 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; 1.3.8.8 [EC: 1.3.8.8]; RXN-17796 [EC: 1.3.8.8, 1.3.8.7]; 1.3.8.- [EC: 1.3.8.8, 1.3.8.7]

Predicted SEED Role

"Acyl-CoA dehydrogenase, short-chain specific (EC 1.3.99.2)" in subsystem Isoleucine degradation (EC 1.3.99.2)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.3.8.7, 1.3.99.-, 1.3.99.2

Use Curated BLAST to search for 1.3.8.7 or 1.3.8.8 or 1.3.99.- or 1.3.99.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (830 amino acids)

>MPMX26_02120 Acyl-coenzyme A dehydrogenase (Acinetobacter radioresistens SK82)
MLFILSILLQLFTIWAIFFYGLSRVTGAITMIGAVIITALISPWSLILGIPLILLSLVLL
VDSLRMNLITRPAFDRLSNAMPSISTTEREALEAGTSWWEKELFMGAPDWDQFDRYPYPH
LSEEEQSFLDHEVEQLCDMLDEWQIHHHDKDLPEHVWQFIKEKGFLGLIIPKIYGGLEFT
AFAQSRIMSKIASRSMTAAVSCMVPNSLGPGELLMHYGTEEQKERYLPGLANGTEIPCFG
LTSPEAGSDAGAIPDTGVVCYDEYDGEQVLGLKMNFSKRWITLAPIATVVGLAFKLFDPD
GLLGDKNKNEYGITCALIPASHPGIEIGPRHYPGSPFMNGTVDGKDVFIPIDWIIGGPKN
AGKGWRMLMECLGVGRGISLPALSTAAGEMCYLTVGAFARIRQQFKLSVGKFEGVQEATS
DIASDAYMLEAFRYMVTCGLNQGGTPAVMTAMAKHYATETMRKVVNHGMDVVGGRAIQLG
PRNFLALQYQAVPVSITVEGANILTRSLMIFGQGSMRCHPYLFEELQLLQSPDKTGALNK
FNEMLYHHLGYTLNRGARALAYAFTGGSSRAAFTADDFTLPYYKLVNRFSACFALTADMS
LGLLAGDIKRKEMLSGRLADIHSYLFIATSILKYYERGNRTEAEQDHAQLALEKAFYQIQ
EAFDGLFANFPVPAAAGLVKMVCFPFGRPVAKPNDKLKQQVAQWIMMENPFREQLKAHVY
YNVREDDVNGRLESTFKMLLQIEPLWDRFKKAESKGQFVGLTFEEHVEDAVSKGFITDEE
AEQLIRYNAKRFDSMLTDNFDMQLKEAFALTNPHLPEHLEVKHEGGMTSV