Protein Info for MPMX26_01466 in Acinetobacter radioresistens SK82

Annotation: Bifunctional protein PutA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF18327: PRODH
  • amino acids 31 to 74 (44 residues), 46.5 bits, see alignment (E = 7e-16)
• PF14850: Pro_dh-DNA_bdg
  • amino acids 86 to 197 (112 residues), 147.1 bits, E=5.6e-47
• PF01619: Pro_dh
  • amino acids 206 to 508 (303 residues), 354.2 bits, E=1.2e-109
• TIGR01238: delta-1-pyrroline-5-carboxylate dehydrogenase
  • amino acids 549 to 1051 (503 residues), 721 bits, E=3.4e-221
• PF00171: Aldedh
  • amino acids 601 to 1047 (447 residues), 370.5 bits, E=2.2e-114

Best Hits

Swiss-Prot: 62% identical to PUTA_SALTY: Bifunctional protein PutA (putA) from Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)

KEGG orthology group: K13821, proline dehydrogenase / delta 1-pyrroline-5-carboxylate dehydrogenase [EC: 1.5.1.12 1.5.99.8] (inferred from 73% identity to acb:A1S_1528)

MetaCyc: 62% identical to trifunctional transcriptional regulator/proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase (Salmonella enterica enterica serovar Typhimurium)
RXN-14903 [EC: 1.5.5.2]; RXN-14116 [EC: 1.5.5.2, 1.2.1.88]

Predicted SEED Role

"Transcriptional repressor of PutA and PutP / Proline dehydrogenase (EC 1.5.99.8) (Proline oxidase) / Delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.5.1.12)" in subsystem Arginine and Ornithine Degradation or Proline, 4-hydroxyproline uptake and utilization or Respiratory dehydrogenases 1 (EC 1.5.1.12, EC 1.5.99.8)

MetaCyc Pathways

• L-proline degradation I (3/3 steps found)
• L-citrulline biosynthesis (6/8 steps found)
• superpathway of L-citrulline metabolism (8/12 steps found)
• L-N^δ-acetylornithine biosynthesis (4/7 steps found)
• L-arginine degradation I (arginase pathway) (1/3 steps found)
• ethene biosynthesis II (microbes) (1/4 steps found)
• (5R)-carbapenem carboxylate biosynthesis (1/6 steps found)

KEGG Metabolic Maps

• Arginine and proline metabolism
• Glutamate metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.2.1.88 or 1.5.1.12 or 1.5.5.2 or 1.5.99.8

Protein Sequence (1250 amino acids)

>MPMX26_01466 Bifunctional protein PutA (Acinetobacter radioresistens SK82)
MNVMDTYSARENAQPHFGYITAFKEKSNFEEQINTAWRRAEPEAVEELLQAAELSAELDQ
KIHELAFNLAHSLRDRKSGSGKAGIVQGLLQEFSLSSQEGIALMCLAEALLRIPDTATRD
LLIRDKINQGNWKEHLGQSNLMFVNAAAWGLMLTGKLMETPTRTSLSSILTGILARSGRG
IIRKAVDVAMRMMGEQFVTGETIGEALNNAKSLEDKGFRYSYDMLGEAALTAEDAERYYD
DYSQAIHAIGQASKDKDVYDGPGISIKLSALHPRYQRAQVIRVHEELYPKILKLAELAKG
YNIGLNIDAEETERLELSLELLERLCFEPSLAQWKGIGFVIQAYQKRCFNLVDYLVDLAK
RSEKRLMIRLVKGAYWDSEIKKAQIEGMSDYPVFTQKVHTDLSYIACAKKLLAAPEQIYP
QFATHNAQTLATIYQLAKPEQYYPGQYEFQCLHGMGEPLYEQVVGSRQDKKLGIPCRIYA
PVGSHETLLAYLVRRLLENGANTSFVNRIADKNLSIESLIRNPVQEIRETAAFTGKLGQK
HPSIPFPQDLYGNLRHNSKGLDLANDQVLKALNDAATRFHDQTWSSQPMLGQEIDSSLER
SLVTVLNPSSHQDIVGYVHEAEAQDVELALTLAQKAESDWANTTPDQRAGMLKKAADLME
ERIQELLVLLSRESGKTYANGLAEVREAVDFLRYYATQMESIAAQSIVTPLGTVLCISPW
NFPLAIFSGQIAAALVAGNCVVAKPAEQTPLIAALAVELLWEAGIPHSVLQLMPGRGETV
GALLSQDARIQGIMFTGSTEVAKILQKTVSGRVTRHGQPVVFIAETGGQNAMIVDSSALT
EQVVLDVLSSAFDSAGQRCSALRILCVQEDNAKPLLHMLKGGMQQLRVGNPVYLNTDIGP
VIDQEAKTNIENHIQKMQKQGYPVYQFAHNPEQNQFKDGTFILPTLIELPNLNELKREVF
GPVLHVITYKQGQLAQLLDAIHAKGYGLTMGLHTRIDETVQTVIQHAEVGNLYINRNIVG
AVVGVQPFGGEGLSGTGPKAGGPLYLYRLMQNCQPKKLEQPFAVQTVNHTSANPLYVDFL
AWVKQNLPQYTLGQEQSFFAGKVFELQGPTGESNQYAVLPRKRTLSLAESEQEQIAQLIA
IFSVGSQPVLLKGNNFYQKHAARLPEVIRQQFHIIENIQTGDFDAVLHHGSQNQLMNLQK
QIAQREGAIVGITHLEPQQLQIPVERLVIERAISINTAAAGGNASLMTLV