Protein Info for MPMX26_01446 in Acinetobacter radioresistens SK82

Annotation: Fumarate hydratase class II

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00979: fumarate hydratase, class II
  • amino acids 4 to 460 (457 residues), 767.7 bits, E=2e-235
• PF00206: Lyase_1
  • amino acids 11 to 341 (331 residues), 386 bits, E=1.5e-119
• PF10415: FumaraseC_C
  • amino acids 407 to 459 (53 residues), 89.9 bits, 1.1e-29

Best Hits

Swiss-Prot: 76% identical to FUMC_NEIMA: Fumarate hydratase class II (fumC) from Neisseria meningitidis serogroup A / serotype 4A (strain Z2491)

KEGG orthology group: K01679, fumarate hydratase, class II [EC: 4.2.1.2] (inferred from 91% identity to aci:ACIAD1890)

MetaCyc: 62% identical to fumarase (Helicobacter pylori 26695)
Fumarate hydratase. [EC: 4.2.1.2]

Predicted SEED Role

"Fumarate hydratase class II (EC 4.2.1.2)" in subsystem TCA Cycle (EC 4.2.1.2)

MetaCyc Pathways

• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle VIII (Chlamydia) (5/6 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• mixed acid fermentation (12/16 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (16/22 steps found)
• TCA cycle III (animals) (7/10 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• reductive TCA cycle I (7/11 steps found)
• incomplete reductive TCA cycle (4/7 steps found)
• superpathway of anaerobic energy metabolism (invertebrates) (11/17 steps found)
• anaerobic energy metabolism (invertebrates, mitochondrial) (5/10 steps found)
• reductive TCA cycle II (6/12 steps found)
• pyruvate fermentation to propanoate I (2/7 steps found)
• methylaspartate cycle (10/19 steps found)
• (S)-lactate fermentation to propanoate, acetate and hydrogen (5/13 steps found)
• L-glutamate degradation VIII (to propanoate) (2/11 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.2

Use Curated BLAST to search for 4.2.1.2

Protein Sequence (464 amino acids)

>MPMX26_01446 Fumarate hydratase class II (Acinetobacter radioresistens SK82)
MQTRIEHDTMGEVEVPNEALWGAQTQRSLQNFKIGQERLPRPMIRAMGLVKKAAAMTNAE
LGQITDEISGYIVGAAEEVIEGKWDAQFPLVVWQTGSGTQSNMNCNEVIANIANQKLGNA
LGSQKPVHPNDHVNRAQSTNDSFPTAIHVAASLQINELLIPAVTRLKDTLERKSKEFKDI
VKIGRTHLQDATPLTLGQEFSGYVSQLDHGLKRLNQALEGLYELPLGGTAVGTGLNAHPQ
YAEKAATRLSQFTGLPFVTAPNKFEALAGRDAAVFASGALKTLATSLNKIANDIRWLASG
PRCGLGELYIPENEPGSSIMPGKVNPTQSEAMTMVVAQVLGNDTTINIAGASGNFELNVF
MPVIAFNLLQSIQLLGDACNSFNDNCAVGIEPNREKIDYFLHNSLMLVTALNPVIGYENA
AKVAKTAYKQGKTLKQVAVELNLVTEQQFDEVVRPEKMVSPNTK