Protein Info for MPMX26_01339 in Acinetobacter radioresistens SK82

Annotation: 50S ribosomal protein L3 glutamine methyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 336 TIGR03533: protein-(glutamine-N5) methyltransferase, ribosomal protein L3-specific" amino acids 18 to 319 (302 residues), 376.8 bits, see alignment E=6.7e-117 TIGR00536: methyltransferase, HemK family" amino acids 20 to 328 (309 residues), 260.4 bits, see alignment E=1.7e-81 PF13489: Methyltransf_23" amino acids 151 to 301 (151 residues), 27.6 bits, see alignment E=1.3e-09 PF02384: N6_Mtase" amino acids 153 to 238 (86 residues), 21.7 bits, see alignment E=7e-08 PF06325: PrmA" amino acids 155 to 232 (78 residues), 32.5 bits, see alignment E=4.2e-11 PF05175: MTS" amino acids 156 to 256 (101 residues), 63.2 bits, see alignment E=1.4e-20 PF09445: Methyltransf_15" amino acids 158 to 234 (77 residues), 24.1 bits, see alignment E=1.4e-08 PF13847: Methyltransf_31" amino acids 159 to 289 (131 residues), 40.8 bits, see alignment E=1.1e-13 PF13649: Methyltransf_25" amino acids 160 to 244 (85 residues), 40 bits, see alignment E=3.1e-13 PF07669: Eco57I" amino acids 198 to 283 (86 residues), 26.4 bits, see alignment E=4.1e-09

Best Hits

KEGG orthology group: K07320, putative adenine-specific DNA-methyltransferase [EC: 2.1.1.72] (inferred from 89% identity to abn:AB57_1922)

Predicted SEED Role

"Protein-N(5)-glutamine methyltransferase PrmB, methylates LSU ribosomal protein L3p"

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.72

Use Curated BLAST to search for 2.1.1.72

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (336 amino acids)

>MPMX26_01339 50S ribosomal protein L3 glutamine methyltransferase (Acinetobacter radioresistens SK82)
MEQSTISPEHLQEAAENLTTIRDFIRFGVSALRQYDAHLGQGTEDYFAESSALVLQTLSL
EWAANPEILDAKLLPSEKEEFLNLLARRVNERIPTSYLLNLAYFFNKPFYVDERVLIPRS
PIAELIENRFAPYCLDEHGQLREAHNNLPVNSDPQTPRRILDMCTGSGCIAIALAYAFPE
AEVDATDISKEALEVAAINTEHHNMQYQVALMESDLFSKIPAENQYDLIVSNPPYVDAED
MADLPEEFLHEPELALAAGQDGLDLVRKMLSQAADYLTEDGLIVIEVGNSEWAMKQNFNT
VDFHWLEFKKGGSGIFALTAEQCRRYQQLFQESVEV