Protein Info for MPMX26_01125 in Acinetobacter radioresistens SK82

Annotation: Glyoxylate/hydroxypyruvate reductase B

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 323 PF00389: 2-Hacid_dh" amino acids 6 to 319 (314 residues), 101.3 bits, see alignment E=5.4e-33 PF02826: 2-Hacid_dh_C" amino acids 109 to 287 (179 residues), 188.8 bits, see alignment E=8.9e-60 PF03807: F420_oxidored" amino acids 149 to 218 (70 residues), 23.1 bits, see alignment E=1.4e-08

Best Hits

Swiss-Prot: 43% identical to GHRB_ESCF3: Glyoxylate/hydroxypyruvate reductase B (ghrB) from Escherichia fergusonii (strain ATCC 35469 / DSM 13698 / CDC 0568-73)

KEGG orthology group: K00090, gluconate 2-dehydrogenase [EC: 1.1.1.215] (inferred from 79% identity to aci:ACIAD1327)

MetaCyc: 51% identical to 2-dehydro-6-phosphogluconate reductase (Pseudomonas fluorescens)
Phosphogluconate 2-dehydrogenase. [EC: 1.1.1.43]

Predicted SEED Role

"Glyoxylate reductase (EC 1.1.1.79) / Glyoxylate reductase (EC 1.1.1.26) / Hydroxypyruvate reductase (EC 1.1.1.81); 2-ketoaldonate reductase, broad specificity (EC 1.1.1.215) (EC 1.1.1.-)" in subsystem Glycolate, glyoxylate interconversions (EC 1.1.1.-, EC 1.1.1.215, EC 1.1.1.26, EC 1.1.1.79, EC 1.1.1.81)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.-, 1.1.1.26, 1.1.1.79, 1.1.1.81

Use Curated BLAST to search for 1.1.1.- or 1.1.1.215 or 1.1.1.26 or 1.1.1.43 or 1.1.1.79 or 1.1.1.81

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (323 amino acids)

>MPMX26_01125 Glyoxylate/hydroxypyruvate reductase B (Acinetobacter radioresistens SK82)
MDKKRVVVFSQIDQTILERLQQQYEVQVLNPKSGDINEQIRQAVTNADGMIGSGRVLNES
NLASAQQLKIISTVSVGYDNYDVDYLNQKKIWLSNTPHVLTETTADLAFSLLMSAARRVP
YLDQWTKQGQWKRTAGTEQFGQDIFGKTLGIIGLGNIGAAVARRGYYGFNMDILYHNRRE
KPELAQPLAAKYCSMSELLQHSDFIVMAVDLNHESKALIGQAEFDQMQSHAVFVNIARGS
VIDEAALINTLKQNKIFAAGLDVYTKEPLQESELFNLSNVVTLPHVGSATEETRKKMAEL
AYQNLVQALEGQIPRYLVNPDFN