Protein Info for MPMX26_01048 in Acinetobacter radioresistens SK82

Annotation: tRNA-specific 2-thiouridylase MnmA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 377 PF02568: ThiI" amino acids 2 to 72 (71 residues), 25.1 bits, see alignment E=3.1e-09 PF03054: tRNA_Me_trans" amino acids 3 to 204 (202 residues), 271.5 bits, see alignment E=1.1e-84 PF02540: NAD_synthase" amino acids 3 to 72 (70 residues), 24.3 bits, see alignment E=4e-09 TIGR00420: tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase" amino acids 3 to 366 (364 residues), 437.6 bits, see alignment E=1.4e-135 PF20259: tRNA_Me_trans_M" amino acids 209 to 277 (69 residues), 70 bits, see alignment E=2.5e-23 PF20258: tRNA_Me_trans_C" amino acids 289 to 366 (78 residues), 77.5 bits, see alignment E=2e-25

Best Hits

Swiss-Prot: 90% identical to MNMA_ACIB3: tRNA-specific 2-thiouridylase MnmA (mnmA) from Acinetobacter baumannii (strain AB307-0294)

KEGG orthology group: K00566, tRNA-specific 2-thiouridylase [EC: 2.8.1.-] (inferred from 91% identity to acd:AOLE_04960)

MetaCyc: 66% identical to tRNA-specific 2-thiouridylase (Escherichia coli K-12 substr. MG1655)
RXN0-2023 [EC: 2.8.1.13]

Predicted SEED Role

"tRNA-specific 2-thiouridylase MnmA"

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.8.1.-

Use Curated BLAST to search for 2.8.1.- or 2.8.1.13

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (377 amino acids)

>MPMX26_01048 tRNA-specific 2-thiouridylase MnmA (Acinetobacter radioresistens SK82)
MQQRVIVGMSGGVDSSVSAALLLQQGYQVEGLFMKNWEEDDGTEYCTAMEDLADAQAVCD
RIGIKLHTANFAMEYWDRVFEHFLAEYAAGRTPNPDILCNKEIKFRAFLDHAVKLGADFI
ATGHYARRGETKYNSQGEAYAELLRGVDTNKDQTYFLHAVHGREINKTLFPVGEIEKPQV
RKIAEELGLATAKKKDSTGICFIGERRFNDFLKQYLPAQPGKIVLDDGKEVGEHHGLMYY
TLGQRGGIGIGGLKGAAEGAWFVLYKDIAGNRLVVGQGHEHPLMQSTILWSEAIDWVAGE
QEIPASGLRCTAKTRYRQADQDCTVYKDDSSTQGVRVEFDDPQRAVTPGQSVVFYSGEVC
LGGGVIHHTNAPVPDFI