Protein Info for MPMX26_00777 in Acinetobacter radioresistens SK82

Annotation: Bifunctional protein FolD protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00763: THF_DHG_CYH
  • amino acids 5 to 120 (116 residues), 137.7 bits, E=2.2e-44
• PF02882: THF_DHG_CYH_C
  • amino acids 127 to 280 (154 residues), 205.3 bits, E=3.6e-65

Best Hits

Swiss-Prot: 93% identical to FOLD_ACIBT: Bifunctional protein FolD (folD) from Acinetobacter baumannii (strain ATCC 17978 / CIP 53.77 / LMG 1025 / NCDC KC755 / 5377)

KEGG orthology group: K01491, methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase [EC: 1.5.1.5 3.5.4.9] (inferred from 92% identity to aci:ACIAD2847)

MetaCyc: 48% identical to methylenetetrahydrofolate dehydrogenase [multifunctional] (Bacillus methanolicus)
Methenyltetrahydrofolate cyclohydrolase. [EC: 3.5.4.9]; Methylenetetrahydrofolate dehydrogenase (NADP(+)). [EC: 3.5.4.9, 1.5.1.5]

Predicted SEED Role

"Methylenetetrahydrofolate dehydrogenase (NADP+) (EC 1.5.1.5) / Methenyltetrahydrofolate cyclohydrolase (EC 3.5.4.9)" in subsystem One-carbon metabolism by tetrahydropterines or Serine-glyoxylate cycle or Folate Biosynthesis (EC 1.5.1.5, EC 3.5.4.9)

MetaCyc Pathways

• folate transformations III (E. coli) (8/9 steps found)
• superpathway of tetrahydrofolate biosynthesis and salvage (10/12 steps found)
• formaldehyde oxidation VII (THF pathway) (4/4 steps found)
• folate transformations II (plants) (9/11 steps found)
• tetrahydrofolate salvage from 5,10-methenyltetrahydrofolate (2/2 steps found)
• formate assimilation into 5,10-methylenetetrahydrofolate (2/3 steps found)
• folate transformations I (8/13 steps found)
• reductive glycine pathway of autotrophic CO₂ fixation (5/9 steps found)
• L-histidine degradation III (1/6 steps found)
• reductive acetyl coenzyme A pathway I (homoacetogenic bacteria) (2/10 steps found)
• purine nucleobases degradation I (anaerobic) (5/15 steps found)
• purine nucleobases degradation II (anaerobic) (10/24 steps found)
• Methanobacterium thermoautotrophicum biosynthetic metabolism (20/56 steps found)

KEGG Metabolic Maps

• Glyoxylate and dicarboxylate metabolism
• One carbon pool by folate

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.5.1.5 or 3.5.4.9

Protein Sequence (282 amino acids)

>MPMX26_00777 Bifunctional protein FolD protein (Acinetobacter radioresistens SK82)
MALVLDGRTLAKQIEADLLQRVEALKTKTGRTPILATILVGDDGASATYVRMKGNACRRV
GMDSLKVELPKETTTEQLLAEIEKLNENPDVHGILLQHPVPEQIDERACFDAISLEKDVD
GVTCLGFGRMAMGEAAYGSATPAGIMAILKENNIEIAGKHAVVVGRSAILGKPMAMMLLQ
ANATVTICHSRTQNLSEFVKQADILVGAVGKAEFIQKEWIKPGAVVVDAGFHPRDGGGVG
DIQLAGIEELASAYTPVPGGVGPMTITTLIRQTVEAAEKALA