Protein Info for MPMX26_00706 in Acinetobacter radioresistens SK82

Annotation: Siroheme synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 457 TIGR01470: siroheme synthase, N-terminal domain" amino acids 4 to 208 (205 residues), 193.2 bits, see alignment E=4.1e-61 PF13241: NAD_binding_7" amino acids 7 to 116 (110 residues), 88.3 bits, see alignment E=7.2e-29 PF10414: CysG_dimeriser" amino acids 152 to 208 (57 residues), 65.2 bits, see alignment 5.5e-22 TIGR01469: uroporphyrinogen-III C-methyltransferase" amino acids 216 to 451 (236 residues), 321.6 bits, see alignment E=3.6e-100 PF00590: TP_methylase" amino acids 218 to 427 (210 residues), 192.3 bits, see alignment E=1.6e-60

Best Hits

Swiss-Prot: 85% identical to CYSG_ACIAD: Siroheme synthase (cysG) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K02302, uroporphyrin-III C-methyltransferase / precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC: 1.3.1.76 2.1.1.107 4.99.1.4] (inferred from 79% identity to acd:AOLE_03580)

Predicted SEED Role

"Siroheme synthase / Precorrin-2 oxidase (EC 1.3.1.76) / Sirohydrochlorin ferrochelatase (EC 4.99.1.4) / Uroporphyrinogen-III methyltransferase (EC 2.1.1.107)" in subsystem Experimental tye or Heme and Siroheme Biosynthesis or Coenzyme B12 biosynthesis or Dissimilatory nitrite reductase (EC 1.3.1.76, EC 2.1.1.107, EC 4.99.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.3.1.76 or 2.1.1.107 or 4.99.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (457 amino acids)

>MPMX26_00706 Siroheme synthase (Acinetobacter radioresistens SK82)
MDIFPISLKLQQQPCLIVGGGKIAYRKAVLLSQAGAIIDVLAPDIDPALAEIVMQTAGQH
IALSYPADIKLKQYRLVIAATNVAEVNRQVFLDCETNHVLVNSVDDPPHCRFMVPAIIDR
SPLVISIASNGTSPVLSRQIRTQLEAAIPYGMGKLAEFSGKWRSRVKEVIVNPDERRIFW
EELYASPLKEQVFNDRLTDAEQLIEQALSTWKTPKGEVYLVGAGPGDPELLTLKALRLMQ
QADVVIYDRLVSGPILELCRRDAEKVYVGKARSNHAVPQEGINALLVKYAQQGKRVCRLK
GGDPFIFGRGGEEIEELFAAGIAFQVVPGITAASGCSAYAGIPLTHRDYAQSVRFLTGHL
KEGSPELPWSELVYENQTLVLYMGLVGLEKICERLIEHGQRPDMPVALVSKGTTPEQKVV
IGTLADIVSKVSEHQIQAPTLTIIGEVVRLREQLQWQ