Protein Info for MPMX26_00480 in Acinetobacter radioresistens SK82

Annotation: Aconitate hydratase A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 22 to 916 (895 residues), 1424 bits, E=0
• PF00330: Aconitase
  • amino acids 72 to 587 (516 residues), 556.7 bits, E=5.1e-171
• PF00694: Aconitase_C
  • amino acids 716 to 842 (127 residues), 155.1 bits, E=1.3e-49

Best Hits

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 82% identity to abc:ACICU_00562)

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• glyoxylate cycle (6/6 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• superpathway of glyoxylate bypass and TCA (10/12 steps found)
• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (20/26 steps found)
• partial TCA cycle (obligate autotrophs) (7/8 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle I (prokaryotic) (8/10 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• mixed acid fermentation (12/16 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (16/22 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• TCA cycle III (animals) (7/10 steps found)
• TCA cycle VI (Helicobacter) (6/9 steps found)
• TCA cycle VII (acetate-producers) (6/9 steps found)
• reductive TCA cycle I (7/11 steps found)
• reductive TCA cycle II (6/12 steps found)
• methylaspartate cycle (10/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3

Protein Sequence (918 amino acids)

>MPMX26_00480 Aconitate hydratase A (Acinetobacter radioresistens SK82)
MAKYSTINSFNSLTTLKTTKKNYQIFSLRQAQQHLDDFTRLPKSLKILLENLLRFEDQIT
VKQEHIQALVAWQQNRSSVQEIQYRPARVLMQDFTGVPAVVDLAAMRAAVEKAGADPEKI
NPLSPVDLVIDHSVMVDHFADEHAFEANVAVEMQRNGERYQFLRWGQSAFNNFSVVPPGT
GICHQVNLEYLAQTVWTGESDGKEFAFPDTLVGTDSHTTMINGLGVLGWGVGGIEAEAAM
LGQPISMLIPEVMGFQLTGKLREGITATDLVLTITQMLRQKGVVGKFVEFYGDGLAELPL
ADRATIANMAPEYGATCGFFPVDEVTLGYLRLTGRDAERIELVEAYCKEQGLWRETGDEP
VFTDTLTLDMSTVEASLAGPKRPQDRVALSDVPHTFQALMDLHLKPAKAEKERLENEGGG
GTAVEAEQANLPHSNPCCVIEGKNYFLSHGDVVISAITSCTNTSNPSVMLAAGLLAKKAV
EQGLQRKAWVKSSLAPGSKVVTDYLKASGLMPYLDQLGYNLVGYGCTTCIGNSGPLPEPI
EEAIQNFDLNVAAVLSGNRNFEGRVHPLVKTNWLASPPLVIAYGLAGNIRTDLSSEPIGE
GKNGEKIYLKDIWPTQAEIEEALQYVNTQMFHKEYAAVFEGEENWKSLQIPETKTYQWDE
HSTYIRHPPFFEKIDQPPQAVKNIEKARILAVLGDSVTTDHISPAGNIKQDSPAGRYLQE
LGVEPKDFNSYGSRRGNHEVMMRGTFANIRIKNEMLGGEEGGNTLHIPTHKKMSIYDAAM
QYQAENTPLVIIAGKEYGTGSSRDWAAKGTNLLGVKAVIAQSFERIHRSNLVGMGVLPLQ
FMPDESRTSLNINGDEILSIHGLSDDLSPQQILRVELEQPDGTQSSFKVLCRIDTVNEVE
YFKAGGILHYVLRHLIAS