Protein Info for MPMX26_00457 in Acinetobacter radioresistens SK82

Annotation: Aspartate--tRNA(Asp/Asn) ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 592 TIGR00459: aspartate--tRNA ligase" amino acids 1 to 583 (583 residues), 797.5 bits, see alignment E=3.3e-244 PF01336: tRNA_anti-codon" amino acids 19 to 103 (85 residues), 64 bits, see alignment E=2e-21 PF00152: tRNA-synt_2" amino acids 121 to 559 (439 residues), 397.3 bits, see alignment E=9.8e-123 PF02938: GAD" amino acids 311 to 409 (99 residues), 94.9 bits, see alignment E=5.7e-31

Best Hits

Swiss-Prot: 95% identical to SYDND_ACIAD: Aspartate--tRNA(Asp/Asn) ligase (aspS) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K01876, aspartyl-tRNA synthetase [EC: 6.1.1.12] (inferred from 95% identity to aci:ACIAD0609)

MetaCyc: 58% identical to aspartate--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Aspartate--tRNA ligase. [EC: 6.1.1.12, 6.1.1.23]; 6.1.1.12 [EC: 6.1.1.12, 6.1.1.23]

Predicted SEED Role

"Aspartyl-tRNA synthetase (EC 6.1.1.12) @ Aspartyl-tRNA(Asn) synthetase (EC 6.1.1.23)" (EC 6.1.1.12, EC 6.1.1.23)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.12 or 6.1.1.23

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (592 amino acids)

>MPMX26_00457 Aspartate--tRNA(Asp/Asn) ligase (Acinetobacter radioresistens SK82)
MMRTHYCGSLTEAQIDQTVTLCGWVHRRRDHGGVIFLDMRDRDGLVQVVIDPDTPEAFAT
ADKARSEYVLKITGRVRRRYAGTENANMISGQIEVLGKEIEVLAQSETPPFPLNDENTNI
SEEIRLKYRFLDIRRPEMLDRLRFRSKVTNLIRNYLDENGFLDVETPILTRATPEGARDY
LVPSRVSNGSFYALPQSPQLFKQLLMVGGIDRYYQIAKCFRDEDLRADRQPEFTQIDIET
SFMSDDDIMDLMEGMTVKMFDELLGIKFDKFQRMPYSEAMRDYASDKPDLRIPLKLVDVA
DLMQDVEFKVFAGPAKDPKGRIVALRVPGAGSLPRSAIDEYTKFVGIYGAKGLAYIKVNE
LEKGIEGLQSPIVKFIEPIVLDLLKRVGAENGDIVFFGADKAKVVNDAMGALRVKIGHDL
NLATCEWAPLWVVDFPMFEETDDGKWTSVHHPFTLPKSSVEEVKNNPGEALSVAYDMVLN
GTEIGGGSLRIYTLEMQKAIFEALGINEEEAEEKFSFLLNALRYGAPPHGGLAFGLDRLV
MLMTGASSIRDVIAFPKTKTAECPLTQAPAPVEANQLRDLGIRLREQQKKEA