Protein Info for MPMX26_00275 in Acinetobacter radioresistens SK82

Annotation: Polyribonucleotide nucleotidyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 699 transmembrane" amino acids 31 to 47 (17 residues), see Phobius details TIGR03591: polyribonucleotide nucleotidyltransferase" amino acids 12 to 693 (682 residues), 1042.9 bits, see alignment E=0 PF01138: RNase_PH" amino acids 18 to 145 (128 residues), 93.3 bits, see alignment E=4.2e-30 amino acids 326 to 458 (133 residues), 92.8 bits, see alignment E=6.3e-30 PF03725: RNase_PH_C" amino acids 148 to 211 (64 residues), 58.6 bits, see alignment E=1.2e-19 amino acids 462 to 530 (69 residues), 28.4 bits, see alignment E=3.2e-10 PF03726: PNPase" amino acids 243 to 322 (80 residues), 77.3 bits, see alignment E=2.5e-25 PF00013: KH_1" amino acids 559 to 615 (57 residues), 47.5 bits, see alignment 3.2e-16 PF00575: S1" amino acids 621 to 692 (72 residues), 67.6 bits, see alignment E=2.6e-22

Best Hits

Swiss-Prot: 94% identical to PNP_ACIAD: Polyribonucleotide nucleotidyltransferase (pnp) from Acinetobacter baylyi (strain ATCC 33305 / BD413 / ADP1)

KEGG orthology group: K00962, polyribonucleotide nucleotidyltransferase [EC: 2.7.7.8] (inferred from 95% identity to acd:AOLE_17625)

MetaCyc: 62% identical to polynucleotide phosphorylase (Escherichia coli K-12 substr. MG1655)
Exoribonuclease II. [EC: 3.1.13.1]; Polyribonucleotide nucleotidyltransferase. [EC: 3.1.13.1, 2.7.7.8]

Predicted SEED Role

"Polyribonucleotide nucleotidyltransferase (EC 2.7.7.8)" in subsystem Bacterial RNA-metabolizing Zn-dependent hydrolases or Polyadenylation bacterial (EC 2.7.7.8)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.13.1

Use Curated BLAST to search for 2.7.7.8 or 3.1.13.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (699 amino acids)

>MPMX26_00275 Polyribonucleotide nucleotidyltransferase (Acinetobacter radioresistens SK82)
MSMFNIVRKEFQFGQHQVVLETGRVARQANSVLITMGGVSVLVAVVAQPTAKAGQDFFPL
TVNYQEKQYAAGRIPGGYGKREGRASEAETLISRLIDRPIRPLFPEGYYNEIQVTATVVS
SDKTMDADIAAMLGTSAALSIAGTPFRGPIAGARVGLINGEYILNPNHEQLKESDLDLVV
AGTETAVLMVESEAKELSEDQMLGAVLFGHDEMQIAIQAIKEFAAAAGATESTWVAPTKN
DALLEQLKTAFEAKISEAYTIAVKQERYAALDALHAEAIAQFVPESDETGIADEVNELFE
ELKYRTVRDNILSGKPRIDGRDTKTVRALDVQVGVLERAHGSALFTRGETQALVTATLGN
TRDALMVDTLSGTKTDNFMLHYNFPAYSVGETGRESGPKRREIGHGRLARRGVQAVLPPV
DRFPYVLRVVSDITESNGSSSMASVCGASLALMDAGVPLKAPVAGIAMGLVKEGERFAVL
SDILGDEDHLGDMDFKVAGSANGITALQMDIKIEGITEEIMEVALNQAFTGRMHILNEMN
KVISRARPEISMHAPTFAVITINPDKIRDVIGKGGATIRQITEETKAAIDIEDNGTVRVF
GETKAAANAAIAKIQALTAEVEPGKIYDGKVIRIVEFGAFVNIMPGTDGLLHISQISNER
IANVTDVLKEGQEVKVQVADVDNRGRIKLTMKDIEQSSN