Protein Info for MPMX19_06963 in Azospirillum sp. SherDot2

Annotation: (Z)-2-((N-methylformamido)methylene)-5- hydroxybutyrolactone dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00171: Aldedh
  • amino acids 12 to 477 (466 residues), 584.7 bits, E=5.9e-180

Best Hits

Swiss-Prot: 44% identical to ALDH4_BACSU: Putative aldehyde dehydrogenase DhaS (dhaS) from Bacillus subtilis (strain 168)

KEGG orthology group: K00128, aldehyde dehydrogenase (NAD+) [EC: 1.2.1.3] (inferred from 67% identity to reh:H16_A1495)

MetaCyc: 44% identical to 4-hydroxymuconic-semialdehyde dehydrogenase (Pseudomonas sp. ENV2030)
4-hydroxymuconic-semialdehyde dehydrogenase. [EC: 1.2.1.61]

Predicted SEED Role

"Aldehyde dehydrogenase (EC 1.2.1.3)" in subsystem Entner-Doudoroff Pathway or Glycerolipid and Glycerophospholipid Metabolism in Bacteria or Methylglyoxal Metabolism or Pyruvate metabolism II: acetyl-CoA, acetogenesis from pyruvate (EC 1.2.1.3)

MetaCyc Pathways

• ethanol degradation II (3/3 steps found)
• ethanol degradation IV (3/3 steps found)
• hypotaurine degradation (3/3 steps found)
• superpathway of NAD/NADP - NADH/NADPH interconversion (yeast) (6/8 steps found)
• phytol degradation (3/4 steps found)
• superpathway of glycol metabolism and degradation (5/7 steps found)
• ethanol degradation III (2/3 steps found)
• putrescine degradation IV (2/3 steps found)
• ethylene glycol degradation (1/2 steps found)
• putrescine degradation I (1/2 steps found)
• putrescine degradation V (1/2 steps found)
• Entner-Doudoroff pathway II (non-phosphorylative) (6/9 steps found)
• 4-nitrophenol degradation I (3/5 steps found)
• mitochondrial NADPH production (yeast) (3/5 steps found)
• D-arabinose degradation II (2/4 steps found)
• fatty acid α-oxidation I (plants) (2/4 steps found)
• histamine degradation (1/3 steps found)
• 3-methyl-branched fatty acid α-oxidation (3/6 steps found)
• 4-hydroxyacetophenone degradation (2/5 steps found)
• octane oxidation (2/5 steps found)
• L-tryptophan degradation X (mammalian, via tryptamine) (1/4 steps found)
• putrescine degradation III (1/4 steps found)
• serotonin degradation (3/7 steps found)
• superpathway of L-arginine and L-ornithine degradation (7/13 steps found)
• dopamine degradation (1/5 steps found)
• superpathway of ornithine degradation (3/8 steps found)
• superpathway of L-arginine, putrescine, and 4-aminobutanoate degradation (5/11 steps found)
• ceramide degradation by α-oxidation (2/7 steps found)
• sphingosine and sphingosine-1-phosphate metabolism (4/10 steps found)
• alkane oxidation (1/6 steps found)
• limonene degradation IV (anaerobic) (1/7 steps found)
• aromatic biogenic amine degradation (bacteria) (1/8 steps found)
• noradrenaline and adrenaline degradation (4/13 steps found)
• ceramide and sphingolipid recycling and degradation (yeast) (4/16 steps found)
• superpathway of pentose and pentitol degradation (14/42 steps found)

KEGG Metabolic Maps

• 1,2-Dichloroethane degradation
• 3-Chloroacrylic acid degradation
• Arginine and proline metabolism
• Ascorbate and aldarate metabolism
• Butanoate metabolism
• Fatty acid metabolism
• Glycerolipid metabolism
• Glycolysis / Gluconeogenesis
• Histidine metabolism
• Limonene and pinene degradation
• Lysine degradation
• Propanoate metabolism
• Pyruvate metabolism
• Tryptophan metabolism
• Urea cycle and metabolism of amino groups
• Valine, leucine and isoleucine degradation
• beta-Alanine metabolism
• gamma-Hexachlorocyclohexane degradation

Isozymes

Compare fitness of predicted isozymes for: 1.2.1.3

Use Curated BLAST to search for 1.2.1.3 or 1.2.1.61

Protein Sequence (492 amino acids)

>MPMX19_06963 (Z)-2-((N-methylformamido)methylene)-5- hydroxybutyrolactone dehydrogenase (Azospirillum sp. SherDot2)
MERYKLFIDGDWCDPVSNEWFETTEPFSGRAWAEIPRGTQADADRAVEAAHRAFLSPDWA
GLTATQRGALLRRLAGLIEDNVDRLGAIEQRDNGKLMAEVSGQVRNVAQWFHYYAGLADK
VQGAVVPINKADVFNYVKYEPLGVVVAITPWNSPLALTTWKMAPALAAGNTIVIKPSEYT
SASILELAKLAHEAGFPRGVVNVVTGFGGEVGEPLVRHPLTAKIAFTGGDIGGQRVNEAA
APGLKKVTLELGGKSPNIVFDDADLDQAVKGAISGVFGASGQTCMAGSRLLVQKTIHDAF
VAKLVAAAGEARIGDPSSMDTQIGPIATRPQWEKILRMIDMAKQEGAVCALGGHALSGPD
YGQGQFVAPTIFTQVRNDMRIAQEEVFGPVLCVIPFEDEDDALRIANDVEFGLAAGVWTR
DLHRAMYCVDRLRAGTVWVNNYRSTSFTTPFGGYKRSGLGREGGVEAIKEYLQTKSVWIT
TKPNRANPFVLG