Protein Info for MPMX19_06941 in Azospirillum sp. SherDot2
Annotation: putative acyl-CoA dehydrogenase fadE25
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 33% identical to CH1CO_SYNAS: Cyclohex-1-ene-1-carbonyl-CoA dehydrogenase (SYN_02587) from Syntrophus aciditrophicus (strain SB)
KEGG orthology group: None (inferred from 74% identity to bbr:BB0656)MetaCyc: 33% identical to cyclohex-1-ene-1-carbonyl-CoA dehydrogenase monomer (Syntrophus aciditrophicus)
R265-RXN [EC: 1.3.8.10]; 1.3.8.10 [EC: 1.3.8.10]
Predicted SEED Role
"Butyryl-CoA dehydrogenase (EC 1.3.99.2)" in subsystem Acetyl-CoA fermentation to Butyrate or Anaerobic respiratory reductases or Butanol Biosynthesis or Isobutyryl-CoA to Propionyl-CoA Module or Isoleucine degradation or Valine degradation (EC 1.3.99.2)
MetaCyc Pathways
- 1-butanol autotrophic biosynthesis (engineered) (21/27 steps found)
- pyruvate fermentation to hexanol (engineered) (8/11 steps found)
- pyruvate fermentation to butanol II (engineered) (4/6 steps found)
- benzoyl-CoA degradation III (anaerobic) (2/9 steps found)
- crotonate fermentation (to acetate and cyclohexane carboxylate) (5/16 steps found)
- benzoate fermentation (to acetate and cyclohexane carboxylate) (5/17 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.3.99.2
Use Curated BLAST to search for 1.3.8.10 or 1.3.99.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (388 amino acids)
>MPMX19_06941 putative acyl-CoA dehydrogenase fadE25 (Azospirillum sp. SherDot2) MQMPIGLWTDPVEPEFLAPLRRAVADHVEPDADAIDREDVYPVAAIKELARQGYTSLTLP EEYGGRGLGFAHCAALFEEASYASAAVGISLITILQAQTIIRLFGADSLKRTVLPEFGRG LITSYALTEANHGSDIRSLDTKAVRDGDRWILSGRKSFITSGSAAEAYVILAETPVGVSA FFVRQGMDGVSTEEGPNAATFGLRNGPHVDLVLDRVTLPADHLIGVEGKGVRQAVTTLDY SRTMAAAISLGIARAAFDGALDFAAGRSAFDRRILDFQGIQWYFADMLADIDAARLLTYE AARKLDAHDDIARFGSEAKLLASRVATDTAARAVQICGAYGVMESARFGRYLRDAKAYEI AGGSSEILKNTIAKRLQQHVALRREALS