Protein Info for MPMX19_06734 in Azospirillum sp. SherDot2

Annotation: Alginate biosynthesis protein AlgA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 488 TIGR01479: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase" amino acids 9 to 487 (479 residues), 702.9 bits, see alignment E=1e-215 PF00483: NTP_transferase" amino acids 11 to 303 (293 residues), 187.4 bits, see alignment E=6.8e-59 PF22640: ManC_GMP_beta-helix" amino acids 318 to 365 (48 residues), 45.7 bits, see alignment 9.8e-16 PF01050: MannoseP_isomer" amino acids 369 to 483 (115 residues), 186.3 bits, see alignment E=3.5e-59 PF07883: Cupin_2" amino acids 401 to 467 (67 residues), 42.1 bits, see alignment E=1.2e-14

Best Hits

Swiss-Prot: 59% identical to XANB_XANCP: Xanthan biosynthesis protein XanB (xanB) from Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25)

KEGG orthology group: None (inferred from 94% identity to azl:AZL_f01870)

MetaCyc: 57% identical to mannose-1-phosphate guanylyltransferase (Escherichia coli K-12 substr. MG1655)
Mannose-1-phosphate guanylyltransferase. [EC: 2.7.7.13]

Predicted SEED Role

"Mannose-1-phosphate guanylyltransferase (GDP) (EC 2.7.7.22)" in subsystem Alginate metabolism or Mannose Metabolism (EC 2.7.7.22)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.7.7.13 or 2.7.7.22

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (488 amino acids)

>MPMX19_06734 Alginate biosynthesis protein AlgA (Azospirillum sp. SherDot2)
MNGNSAQKIVPVLLSGGTGSRLWPLSRELYPKQFLPLCSDRTMLQDTALRVSAQPGSPAA
AAAEAVFAAPLVICNQEHRFLAAEQLRQSRCSPRGIILEPAGRNTAAACALAALAVAADD
PDALLLILPADHEIRDAEAFRRAVGIAARAAQAGRLVTFGIAPTRPETGYGYIRRGHSMD
EADGAYNVAAFVEKPSLEVAERYLAEGTYAWNSGMFLFPASRLLAELERHAPAVLDACRE
ALEQGSKDLDFFRLDAAAFAKAPSISIDYAVMERTDRAAVVPCEIGWTDVGAWSALWDVG
NKDGEGNVIVGDVLLEGARDCYVRSENHLTAVVGVENAVVVVVDDAVLVADRSQAQDVKR
IVDRLKAEGRSESVSHRRVHRPWGSYQSLHTGDRFQVKSLTIAPGSRLSLQKHHHRAEHW
VVVNGTALVTRGEEQVMVYENQSIYIPIGTVHRLENPGKVPLTIIEVQSGSYLGEDDIVR
LEDVYGRN