Protein Info for MPMX19_06430 in Azospirillum sp. SherDot2

Annotation: Copper-exporting P-type ATPase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 812 transmembrane" amino acids 174 to 195 (22 residues), see Phobius details amino acids 201 to 220 (20 residues), see Phobius details amino acids 232 to 254 (23 residues), see Phobius details amino acids 265 to 283 (19 residues), see Phobius details amino acids 417 to 439 (23 residues), see Phobius details amino acids 445 to 465 (21 residues), see Phobius details amino acids 761 to 780 (20 residues), see Phobius details amino acids 786 to 805 (20 residues), see Phobius details TIGR00003: copper ion binding protein" amino acids 20 to 82 (63 residues), 46.9 bits, see alignment 6.3e-16 amino acids 89 to 149 (61 residues), 34.7 bits, see alignment 3.9e-12 PF00403: HMA" amino acids 22 to 82 (61 residues), 59.1 bits, see alignment 9e-20 amino acids 90 to 148 (59 residues), 60 bits, see alignment 4.7e-20 TIGR01511: copper-translocating P-type ATPase" amino acids 216 to 805 (590 residues), 584.1 bits, see alignment E=8.3e-179 TIGR01525: heavy metal translocating P-type ATPase" amino acids 234 to 804 (571 residues), 633.1 bits, see alignment E=1.3e-193 TIGR01494: HAD ATPase, P-type, family IC" amino acids 273 to 532 (260 residues), 161.9 bits, see alignment E=4.7e-51 amino acids 619 to 786 (168 residues), 147 bits, see alignment E=1.5e-46 PF00122: E1-E2_ATPase" amino acids 300 to 480 (181 residues), 204 bits, see alignment E=2.9e-64 PF00702: Hydrolase" amino acids 497 to 716 (220 residues), 114.4 bits, see alignment E=1.8e-36

Best Hits

KEGG orthology group: K01533, Cu2+-exporting ATPase [EC: 3.6.3.4] (inferred from 58% identity to ppg:PputGB1_0631)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (812 amino acids)

>MPMX19_06430 Copper-exporting P-type ATPase (Azospirillum sp. SherDot2)
MADAVAREWTGPQELAASEIDITVGGMTCASCVARVERAIRKVPGVADVSVNLATEKARI
SFADSVPNTLGVVEAIEKAGYEPATTEFDLRVEGMTCASCVGRVEKALRKVPGVSDAALN
LATERAHVTGHGIDSSTLAAAIEKAGYQARPATEGTTTASDEAQDRSRKELQHVFIGAVL
SLPLVVGMVGDLLGYDVMPPGWLQFLLATPVQFWLGWRFYRAAYRAVRAGAGNMDLLVAI
GTTASWGVSIYMLLTAHPGHGMPHLYFEGSAVLITFVLLGKWLETRAKGQTAVAIRALMS
LRPDTARVRRGAAEIEVPVGQVRRGDLVVVRPGERIPVDGRVVEGTGSVDESMLTGEPLP
VEKAAGAKVTGGSINVDGLLMVETTAVGAETMLAKIVRMVEGAQASKAPIQRTVDRVSAI
FVPVVLLIAIVTFVAWWGFTGNVEAAIVTAVSVLVIACPCALGLATPTSIMVGTGAAARH
GILIKDAEALERAHAVTTVAFDKTGTLTEGKPMVTNVSPAADQLRTEVLRLAATLQQGSE
HPLARSMRKRTSVEGITAAALSDFKALPGRGISGIVEGRTPQLGSRRLAAENGHAEHALE
VSAADWEKSGQTVSWLFELAPERRVLGIVGFGDTVKEAARKAVQKLHEQGIEAVMVTGDS
KGAANAVARELGIDRVFAEVLPGDKAEVVASLKREGKVVAMVGDGINDAPALASADVGIA
MGTGTDVAMATAGVTLMRGNPVLVGGAVDVSRRTYAKIKQGLFWAFAYNVISIPLAALGY
LSPVLAGAAMALSSVSVVLNALTLRGWKPRGI