Protein Info for MPMX19_06335 in Azospirillum sp. SherDot2

Annotation: Arsenite oxidase subunit AioA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 822 TIGR02693: arsenite oxidase, large subunit" amino acids 10 to 802 (793 residues), 1213.5 bits, see alignment E=0 PF18465: Rieske_3" amino acids 24 to 119 (96 residues), 126 bits, see alignment E=8.8e-41 PF00384: Molybdopterin" amino acids 123 to 577 (455 residues), 89.5 bits, see alignment E=3.4e-29 PF01568: Molydop_binding" amino acids 692 to 790 (99 residues), 49 bits, see alignment E=8.9e-17

Best Hits

Swiss-Prot: 51% identical to AIOA_ALCFA: Arsenite oxidase subunit AioA (aioA) from Alcaligenes faecalis

KEGG orthology group: K08356, arsenite oxidase large subunit [EC: 1.20.98.1] (inferred from 66% identity to sno:Snov_1288)

MetaCyc: 51% identical to arsenite oxidoreductase large subunit (Alcaligenes faecalis)
1.20.98.1-RXN [EC: 1.20.9.1]; RXN-12586 [EC: 1.20.9.1, 1.20.2.1]

Predicted SEED Role

"Assimilatory nitrate reductase large subunit (EC:1.7.99.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.99.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.20.9.1, 1.20.98.1, 1.7.99.4

Use Curated BLAST to search for 1.20.2.1 or 1.20.9.1 or 1.20.98.1 or 1.7.99.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (822 amino acids)

>MPMX19_06335 Arsenite oxidase subunit AioA (Azospirillum sp. SherDot2)
MTYKTGIDQLPIPPKDAKVQNVTCDFCIVGCGYKAISWPIDRQGGAEAGDNVFGEDLSQQ
QGAESPAWYNPSMYNVVQQDGRLVHLIVKPDKACEVNAGLNSVRGGRMGVTRYSEQTGTM
TARLTQPMVWRYSQMMPSSWDDSLSLVADVTRRVIEEMGEDGVIVSAFDHGGAAGGYENT
WGTGKLYFDAMKVKNIRIHNRPAYNSEVHASRDMGVGELNNSYEDAELADTIFVVGANPL
ENQTNYFLAHWVPNLRGETLDKKRQMLASEAHAPARIIIVDPRRTVSVNACEAVAGKENV
LHLAIAPGTDLVLLNALLTEVVNKGWHAKDFIAQHTAGFEDAVAANRISVEDAAAVTGLA
PADIRKAAQWIAEPKAGGTQRRTMHAYEKGLIWGNDNYRTNGAMVNLALATWNVGREGTG
CVRLGGHQEGYVRPSDAHVGRPAAYVDQLLISGQGGVHHVWATDHFKSTLNAEEFRRIYR
RRTNLVKEAMDSVPYGDRQAQLEQIVAAIKRGGLFSVVVDILPTQIGEAAHVWLPAATAG
EMNLTSMNGERRLRLTERYMDPPGVAMPDALIAARMANAIEASFRKAGNGAYADQFKGKF
DWKTEEDAFMDGYHEHAEGGTFVTYDRLRNMGTNGVHEPVVGFENGRLIGTKRLYTDGKF
KTADGRAKFMKTEWRGLQVAGREQQREKYAFLINNGRNNLVWQNAFYDQHTPFVRERMPV
APIEMNPGDMTTLGLQAGDLVEVYNDVGSTQAMVYPTPAAKPGQAFMLFIAPTGQVGNVI
SKATNELMIPDYKNVWANIRRIGHVSSADGMSFKNIEYPQAL